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A multipurpose broad host range plasmid, pIJ3200, was constructed by inserting the polylinker-containing 445 by PvuII fragment of Bluescript M13 into the EcoRI site of the cosmid pLAFR1. Using this(More)
A real-time assay system that allows monitoring of intracellular human enterovirus (HEV) protease activity was established using the principle of fluorescence resonance energy transfer (FRET). It was(More)
AIM To observe the effect and mechanism of scopolamine on morphine(Mor)-induced mice dependence. METHODS The Mor-dependent mice model was established by intraperitoneal (ip) administered Mor for(More)