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BACKGROUND A sequenced house dust mite (HDM) genome would advance our understanding of HDM allergens, a common cause of human allergies. OBJECTIVE We sought to produce an annotated Dermatophagoides farinae draft genome and develop a combined genomic-transcriptomic-proteomic approach for elucidation of HDM allergens. METHODS A D farinae draft genome and(More)
Due to the irregular of diet and overfeeding greasy and surfeit flavor closely associated with hyperuricemia disease, the lipid emulsion containing high cholesterol was used to model. To obtain a more stable and sustained animal model for the efficacy evaluation of traditional Chinese herbs, we observed the influence on the serum uric acid of rat induced by(More)
BACKGROUND House dust mites (HDMs) are the major sources of indoor allergens which induce asthma, dermatitis, rhinitis, and some other allergic diseases. Close to 30 sub-allergens have been identified. METHODS Through analyzing the full genome sequence of dust mite, a new allergen whose primary structure belongs to the heat shock protein family was(More)
OBJECTIVE To study the chemical constituents of Juncus setchuensis. METHODS Column chromatography was used in the isolation procedure. The structures of isolated compounds were elucidated by spectral data. RESULTS Eight compounds were isolated and their structures were identified as 2-hydroxy-3-methylanthraquinone (1), physcion (2), stigmasterol (3),(More)
Recombinant proteins expressed by prokaryotic expression system are normally in the form of inclusion. In the present paper, refolding process of recombinant pan-allergen profilin protein induced by urea has been investigated by using circular dichroism spectra, fluorescence spectra, synchronous fluorescence spectra systematically. And the spectral(More)
OBJECTIVE To investigate the compliance of standard antenatal care (ANC) model with 12-13 visits currently used in Beijing region, and to assess the efficacy of this model in reducing adverse maternal and perinatal complication. METHODS The clinical data of 544 women who delivered at Peking Union Medical College Hospital (West Section) from January 1,(More)
OBJECTIVE To clone and express Der f7 gene of Dermatophagoides farinae, and identify its immunogenicity. METHODS Total RNA was extracted from D. farinae mites. A reference sequence (Accession No. AY283292) was used to design specific primers. The Der f7 gene fragment was amplified by RT-PCR, and cloned into pET-32a vector. The recombinant plasmid was(More)
OBJECTIVE To compare the macroscopic and microscopic characteristics of Ganoderma and the confusable medicinal mushrooms of "Zhi". METHODS The methods of macroscopic and microscopic examination were employed to authenticate Ganoderma and the other medicinal mushrooms of "Zhi" in the fungal group. RESULTS The medicinal mushroom named "Zhi" could be(More)
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