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Large-conductance Ca(2+)-activated K(+) (BK) channels can regulate cellular excitability in complex ways because they are able to respond independently to two distinct cellular signals, cytosolic Ca(2+) and membrane potential. In rat chromaffin cells (RCC), inactivating BK(i) and noninactivating (BK(s)) channels differentially contribute to RCC action(More)
The structural basis underlying the gating of large conductance Ca(2+)-activated K(+) (BK) channels remains elusive. We found that substitution of Leu-312 in the S6 transmembrane segment of mSlo1 BK channels with hydrophilic amino acids of smaller side-chain volume favored the open state. The sensitivities of channels to calcium and voltage were modified by(More)
Single large-conductance calcium-activated K(+) (BK) channels encoded by the mSlo gene usually have synchronous gating, but a Drosophila dSlo (A2/C2/E2/G5/10) splice variant (dSlo1A) exhibits very flickery openings. To probe this difference in gating, we constructed a mutant I323T. This channel exhibits four subconductance levels similar to those of dSlo1A.(More)
To allocate cloud resources efficiently and obtain the maximum economic benefit are the major goals of the cloud resource providers and users. The objective of this paper is to present a novel cloud resource allocation algorithm named NECDA to overcome some of the shortcomings of the current mechanisms. A cloud resource allocation model of m*n type based on(More)
—According to the basic features of high resolution remote sensing images, a novel edge detection algorithm based on wavelet enhancement and mathematical morphology is proposed. First, the remote sensing image is decomposed by a wavelet transform to get the low frequency part and high frequency part. Then a Laplacian sharpening operation is doing on the low(More)
A code is said to be a w-identifiable parent property code (or w-IPP code for short) if whenever d is a descendant of w (or fewer) codewords, one can always identify at least one of the parents of d. Let C be an (N,w+1,q)-code and C * an (w+1)-color graph for C. If a graph G is a subgraph of C * and consists of w+1 edges with different colors, then G is(More)
FLT3 has been identified as a valid target for the treatment of acute myeloid leukemia (AML), and some FLT3 inhibitors have shown very good efficacy in treating AML in clinical trials. Nevertheless, recent studies indicated that relapse and drug resistance are still difficult to avoid, and leukemia stem cells (LSCs) are considered one of the most important(More)
To study the role of the thyroid hormone (TH) in cerebellar development, we generated transgenic mice expressing a dominant-negative TH receptor (TR) in cerebellar Purkinje cells. A mutant human TRβ1 (G345R), which binds to the TH-response element but cannot bind to T3, was subcloned into exon 4 of the full-length L7/Pcp-2 gene, which is specifically(More)
Glioblastoma multiforme (GBM) is the prevalent and most fatal brain tumor in adults. Invasion and a high rate of recurrence largely contribute to the poor prognosis of GBM. The current standard therapy for GBM includes surgery with maximum feasible resection, radiotherapy, and treatment with chemotherapeutic agent temozolomide. Annexin A5 reportedly(More)