Yu. S. Massino

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Russian], Moscow (1983), pp. 226-227. 7. A. A. Yushchenko, Current Problems in Leprology [in Russian], Astrakhan' (1984), pp. 4042. 8. A. A. Yushchenko and F. E. Vishnevetskii, Byull. ~ksp. Biol. Med., No. 3, 376 (1987). 9. J. Convit, N. Aranzazu, and M. E. Pinardi, The Armadillo as an Experimental Model in Biomedical Research, Washington (1976), pp. 41-46.(More)
The dependence of the antigen-binding activity of immobilized antibodies on pH of a saturating buffer has been investigated. We analyzed 28 monoclonal antibodies (MCAs) produced by various hybridomas to three virus antigens, i.e., the nuclear p23 protein of hepatitis C virus (C core protein p23), p24 protein of HIV 1, and the surface antigen of hepatitis B(More)
  • Yu. S. Massino, B. P. Kopnin
  • 2004
Resistance of tumor cells to the antitumor agent adriablastin (synonym: adriamycin) arises in some cases as a result of a decrease in accumulation of the drug by resistant cells [i0, ii]. Cells resistant to adriablastin acquire cross-resistance to a number of agents differing in their chemical structure and mechanism of action: actinomycin D, vincristine,(More)
The change in the concentration and antigen-binding activity of 28 monoclonal antibodies was studied after their adsorption on the surface of polystyrene microplates in buffers with different pH values (1.0, 2.8, 7.5, 9.6, and 11.9). We used 16 clones to the HIV p24 protein and 12 clones to the surface antigen of Hepatitis B Virus. The binding efficiency of(More)
The antigen-binding capacities of chicken and goat polyclonal antibodies and mouse monoclonal antibodies specific for the hepatitis B virus surface antigen were compared via enzyme immunoassay. It was shown that the use of both adsorbed and detecting chicken antibodies reduces significantly the analytical sensitivity of the sandwich method for the detection(More)
Bispecific antibodies are purified from ascitic fluid by successive affinity chromatography on HRP-Sepharose and IgG-Sepharose. The ability of the affinity purified bispecific antibodies to bind human IgG and horseradish peroxidase is demonstrated in the immunoenzyme assay (identification of anti-HIV antibodies.
One of the most commonly used methods of obtaining bispecific monoclonal antibodies (McAb) is by the fusion of two hybridomas, secreting antibodies to different antigens. Under these circumstances hybrid (tetradoma) clones secreting recombinant immunoglobulins may be formed [14]. As a rule, selective markers are introduced beforehand into one of the(More)
Along with common monoclonal antibodies hybridoma technology helps obtain bifunctional antibodies carrying binding sites for two different antigens. For this purpose two hybridomata forming different monoclonal antibodies are usually fused together. As a result of recombination of the light and heavy chains of parent Ig in hybrid hybridomata (quadromata),(More)
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