Yu. S. Massino

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The antigen-binding capacities of chicken and goat polyclonal antibodies and mouse monoclonal antibodies specific for the hepatitis B virus surface antigen were compared via enzyme immunoassay. It was shown that the use of both adsorbed and detecting chicken antibodies reduces significantly the analytical sensitivity of the sandwich method for the detection(More)
Along with common monoclonal antibodies hybridoma technology helps obtain bifunctional antibodies carrying binding sites for two different antigens. For this purpose two hybridomata forming different monoclonal antibodies are usually fused together. As a result of recombination of the light and heavy chains of parent Ig in hybrid hybridomata (quadromata),(More)
Resistance of tumor cells to the antitumor agent adriablastin (synonym: adriamycin) arises in some cases as a result of a decrease in accumulation of the drug by resistant cells [i0, ii]. Cells resistant to adriablastin acquire cross-resistance to a number of agents differing in their chemical structure and mechanism of action: actinomycin D, vincristine,(More)
One of the most commonly used methods of obtaining bispecific monoclonal antibodies (McAb) is by the fusion of two hybridomas, secreting antibodies to different antigens. Under these circumstances hybrid (tetradoma) clones secreting recombinant immunoglobulins may be formed [14]. As a rule, selective markers are introduced beforehand into one of the(More)
We have produced a panel of site-specific antibodies recognizing different regions of the human serotonin transporter (SERT). This panel included: 1) monoclonal antibodies 23C5 (mAbs 23C5) to the C-terminal region (amino acid residues 597-630); 2) polyclonal antibodies (pAbs) to the N-terminal region (amino acid residues 69-83); 3) pAbs to the region (amino(More)
The ability of bispecific antibodies (Babs) formed by fusion of hybridomas and parent monoclonal antibodies (Mabs) to interact with the solid phase-adsorbed antigens was studied. Mabs specific to the three different antigens [horseradish peroxidase (HRP), human IgG (hIgG), and human myoglobin (Mb)] as well as Babs with the double specificity(More)
The dependence of the antigen-binding activity of immobilized antibodies on pH of a saturating buffer has been investigated. We analyzed 28 monoclonal antibodies (MCAs) produced by various hybridomas to three virus antigens, i.e., the nuclear p23 protein of hepatitis C virus (C core protein p23), p24 protein of HIV 1, and the surface antigen of hepatitis B(More)
The interaction between two different monoclonal antibodies (Mabs) and their corresponding bispecific antibodies (Babs) with immobilized antigens was investigated using an optical biosensor (IAsys). The analyzed panel of affinity-purified antibodies included two parental Mabs (one of which was specific to human IgG (hIgG), and another one to horseradish(More)
Enzyme-linked sandwich immunosorbent assay for serum myoglobin with 3 monoclonal antibodies to different epitopes is described. Antibodies 13G6 and 18H2 were adsorbed on a carrier, and 14D6 antibodies were labeled with horseradish peroxidase. One-step incubation with standard myoglobin solution yielded double sandwich: adsorbed antibodies—myoglobin—labeled(More)
Bispecific antibodies are purified from ascitic fluid by successive affinity chromatography on HRP-Sepharose and IgG-Sepharose. The ability of the affinity purified bispecific antibodies to bind human IgG and horseradish peroxidase is demonstrated in the immunoenzyme assay (identification of anti-HIV antibodies.