Yu. R. Zakharova

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Determination of the taxonomic position of microorganisms associated with diatoms was carried out using microscopic, microbiological, and phylogenetic methods. Examination of the cultures of the fresh-water diatom Synedra acus grown in Lake Baikal water by epifluorescence and electron microscopy revealed that bacterial cells colonized the mucilage(More)
The specific features of the distribution, abundance, and taxonomic diversity of bacteria oxidizing iron and manganese in the bottom sediments sampled from different areas of Lake Baikal are studied. These bacteria are widespread in Baikal bottom sediments. Cultivated iron- and manganese-oxidizing microorganisms are ascribed to six genera: Metallogenium,(More)
5 An aquaporin like protein of the MIP (major intrinsic protein) family with a molecular weight of approximately 31 kDa was identified in the proteome of the diatom Synedra acus subsp. radians by Western blot hybridization. The analysis of pyrosequencing data showed that the genome of this diatom contained a 858 bp gene encoding a 31.3 kDa protein, which on(More)
High-throughput method of sequencing was applied to determine the complete nucleotide sequence of an araphid pennate diatom Synedra acus subsp. radians from Lake Baikal (East Siberia). The assembled genome has a total length of 98 Mbp, the mean coverage is 33x. Structure-functional annotation of the genome was performed.
The existence of the cluster of duplicated sit silicon transporter genes in the chromosome of the diatom Synedra acus subsp. radians was shown for the first time. Earlier, the localization of sit genes in the same chromosome and cluster formation caused by gene duplication was shown only for the marine raphid pennate diatom P. tricornutum. Only(More)
Taxonomic diversity of Lake Baikal bacteria during the period of massive under-ice development of dinoflagellate Gymnodinium baicalense was studied. During the ice-covered period in 2013, both the abundance and biomass of G. baicalense were several orders of magnitude higher than the values for previous years, the maximum values were 8.9 × 106 cells/L and(More)
Pyrosequencing of amplicons V3-V4 of the 16S rRNA gene of 30 samples from the photic layer across the three basins of Lake Baikal has revealed 44 958 sequences of the domain Bacteria. The most representative phylums among all classified sequences in the samples were Actinobacteria, Bacteroidetes, Verrucomicrobia, Proteobacteria, Acidobacteria, and(More)
Methods for cultivation of microorganisms oxidizing iron and manganese in bottom sediments of Lake Baikal were tested. Bacteria were grown on selective media with a characteristic accumulation of metal oxides. The morphology and developmental patterns of the isolated microbial group were studied. The studied sediment core was used to isolate 42 strains of(More)
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