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During the past two decades, there have been numerous attempts at using animals in order to produce recombinant human proteins and monoclonal antibodies. However, it is only recently that the first two therapeutic agents isolated from the milk of transgenic animals, C1 inhibitor (Ruconest) and antithrombin (ATryn), appeared on the market. This inspires hope… (More)
Expression in milk of transgenic farm animals is one of the most effective ways to produce eukaryotic recombinant proteins. In this review, we discuss the problems of the appropriateness of selecting this expression system for the production of proteins and the development characteristics of appropriate production processes.
Postnatal development of mammalian oocytes is accompanied by functional and structural remodeling of the nucleolar apparatus: the final stage of this process is the formation of large objects (up to 10 μm in diameter) termed nucleolus-like bodies (NLBs) in preovulatory GV oocytes. NLB material was shown to be essential for early embryonic development, but… (More)
In the present work, we studied the state of chromosomal nucleolar organizing regions (NOR) in hybrid cells obtained by fusion of cells of embryonal carcinoma of a murine line PCC4aza1 and of cells of adult mouse spleen at cultivation of hybrids under different conditions. The obtained results have shown that long-term cultivation of hybrid cells in a… (More)