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BACKGROUND The presence of fetal DNA in maternal plasma represents a source of fetal genetic material for noninvasive prenatal diagnosis; however, the coexisting background maternal DNA complicates the analysis of aneuploidy in such fetal DNA. Recently, the SERPINB5 gene on chromosome 18 was shown to exhibit different DNA-methylation patterns in the(More)
BACKGROUND The discovery of cell-free fetal DNA in maternal plasma has opened up new possibilities for noninvasive prenatal diagnosis. However, the use of maternal plasma fetal DNA for the direct detection of fetal chromosomal aneuploidies has not been reported. We postulate that the aneuploidy status of a fetus could be revealed by an epigenetic allelic(More)
BACKGROUND The availability of an early diagnostic tool for severe acute respiratory syndrome (SARS) would have major public health implications. We investigated whether the SARS coronavirus (SARS-CoV) can be detected in serum and plasma samples during the early stages of SARS and studied the potential prognostic implications of such an approach. METHODS(More)
BACKGROUND The use of fetal DNA in maternal plasma for noninvasive prenatal diagnosis of trisomy 21 (T21) is an actively researched area. We propose a novel method of T21 detection that combines fetal-specific epigenetic and genetic markers. METHODS We used combined bisulfite restriction analysis to search for fetal DNA markers on chromosome 21 that were(More)
BACKGROUND The putative promoter of the holocarboxylase synthetase (HLCS) gene on chromosome 21 is hypermethylated in placental tissues and could be detected as a fetal-specific DNA marker in maternal plasma. Detection of fetal trisomy 21 (T21) has been demonstrated by an epigenetic-genetic chromosome dosage approach where the amount of hypermethylated HLCS(More)
Severe acute respiratory syndrome (SARS) is a global health concern. In Hong Kong, two major outbreaks, one hospital based and the other in the Amoy Gardens apartments, were identified. The frequency of diarrhoea, admission to intensive care, and mortality differed significantly between the two outbreaks. We did genomic sequencing for viral isolates from(More)
and the constant bias of Ϫ0.2 mmol/L (Ϫ0.53 to Ϫ0.05) is acceptable. As also shown in Fig. 1, the difference between the two methods is independent of leukocyte concentration up to at least 450 ϫ 10 9 cells/L. White cells, which interfere during spectrophotometric measurement of Hb on automated hematology analyz-ers, are ultrasonically lysed in the(More)
to demonstrate conclusively that SARS-CoV is indeed causative of neurologic manifestations such as those described here and to address the potential neuropathologic se-quelae of SARS-CoV infection of the central nervous system. al. Coronavirus as a possible cause of severe acute respiratory syndrome. Quantitative analysis and prognostic implication of(More)
Male-specific coliphages are often used as indicators of contamination by enteric viruses. These phages can be detected in water samples by plaque assays and by polymerase chain reaction. In this study, the M13 coliphage was used to develop a real-time PCR assay for the detection of male-specific DNA coliphages. The real-time PCR was found to have a(More)
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