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A Novel Method for High-Level Production of TEV Protease by Superfolder GFP Tag
TLDR
A novel method to produce TEV protease by using visible superfolder green fluorescent protein (sfGFP) as the fusion tag and the best variant of sfGFP-TEV fusion protease was obtained with purity of over 98% and yield of over 320 mg per liter culture. Expand
A novel method for high-level production of psychrophilic TAB5 alkaline phosphatase.
TLDR
This research provides a novel method for high-level expression, purification and characterization of TAP which is sufficient for high throughput genome analysis and may replace the widely used shrimp AP because of its low cost. Expand
cAMP/protein kinase A signalling pathway protects against neuronal apoptosis and is associated with modulation of Kv2.1 in cerebellar granule cells
TLDR
The protection against apoptosis via the protein kinase A pathway is associated with a double modulation on IK channel properties and its expression of α‐subunit that is mainly encoded by the Kv2.1 gene. Expand
TR-FRET Assays of Huntingtin Protein Fragments Reveal Temperature and PolyQ Length-Dependent Conformational Changes
TLDR
A reversible temperature dependent change of TR-FRET signals for HTT N-terminal fragments is reported: the signals become higher when the temperature is lowered from room temperature to 4°C, which is potentially useful for understanding polyQ structure and toxicity. Expand
Allele-selective lowering of mutant HTT protein by HTT–LC3 linker compounds
TLDR
Compounds that interact with mutant huntingtin and an autophagosomal protein are able to reduce cellular levels of mutant Huntingtin by targeting it for autophagic degradation, demonstrating an approach that may have potential for treating proteopathies. Expand
Metal chelator EGCG attenuates Fe(III)‐induced conformational transition of α‐synuclein and protects AS‐PC12 cells against Fe(III)‐induced death
  • Juan Zhao, Lihui Xu, +5 authors P. Zhou
  • Chemistry, Medicine
  • Journal of neurochemistry
  • 1 October 2017
TLDR
EGCG significantly inhibits this process by chelating Fe(III) and protects AS‐PC12 cells against the toxicity induced by ROS and β‐sheet‐enriched AS fibrils, yielding useful information that EGCG might be a promising drug to prevent and treat the neurodegenerative diseases. Expand
Associations of protein 4.2 with band 3 and ankyrin
TLDR
The results showed that the CDB3-binding site of protein 4.2 is located in the region of residues 200–211 and the ankyrin-binding Site, which suggested that the Ankyrin D34 domain can interact directly withprotein 4. Expand
Suppression of MAPK11 or HIPK3 reduces mutant Huntingtin levels in Huntington's disease models
TLDR
Two kinases HIPK3 and MAPK11 are reported as positive modulators of mHTT levels both in cells and in vivo, revealing new therapeutic entry points for HD and target-discovery approaches for similar diseases. Expand
(−)-Epigallocatechin-3-gallate (EGCG) inhibits fibrillation, disaggregates amyloid fibrils of α-synuclein, and protects PC12 cells against α-synuclein-induced toxicity
α-Synuclein (α-Syn) aggregates are the major component of Lewy bodies (LB), which is a pathological hallmark in the brain tissue of Parkinson's disease (PD) patients. It has been reported thatExpand
Structure-based engineering of anti-GFP nanobody tandems as ultra-high-affinity reagents for purification
TLDR
This work provides a proof of concept for the design of ultra-high-affinity binders of target proteins through dimerized nanobody chimaeras, and this strategy may also be applied to link interesting target protein nanobodies without overlapping binding surfaces. Expand
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