Yu-Bang Shen

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BACKGROUND De novo transcriptome sequencing is a robust method of predicting miRNA target genes, especially for organisms without reference genomes. Differentially expressed miRNAs had been identified previously in kidney samples collected from susceptible and resistant grass carp (Ctenopharyngodon idella) affected by Aeromonas hydrophila. Target(More)
Pedigree information is essential for the genetic improvement of traits of interests in breeding programs. In this study, twelve microsatellite loci were selected to optimize three multiplex PCR protocols for parentage assignment in grass carp (Ctenopharyngodon idella). One hundred and fifty adult fish and 252 progenies produced from three pilot groups (P1,(More)
MicroRNAs (miRNAs) are endogenous small non-coding RNAs that play crucial roles in numerous biological processes. However, the role of miRNAs in antibacterial defence in fish has not been fully determined. Here, we identified that nine miRNAs are differentially expressed in kidney between susceptible and resistant grass carp strains. Analysis of spatial and(More)
The grass carp (Ctenopharyngodon idella) is an important commercial farmed herbivorous fish species in China, but is susceptible to Aeromonas hydrophila infections. In the present study, we performed de novo RNA-Seq sequencing of spleen tissue from specimens of a disease-resistant family, which were given intra-peritoneal injections containing PBS with or(More)
The grass carp (Ctenopharyngodon idella) is one of the most important freshwater fish species in China. In this study, we reported the isolation and characterization of 25 novel single nucleotide polymorphism (SNP) markers from 47 wild individuals (collected from Yangtze River). The ranges of observed heterozygosity (Ho), expected heterozygosity (He) and(More)
Tilapia is the common name for a group of cichlid fishes. Identification of DNA markers significantly associated with important traits in candidate genes may speed up genetic improvement. l-Amino acid oxidase (LAO) plays a crucial role in the innate immune defences of animals. Previously, whether LAO variants were associated with economic traits had not(More)
Despite the importance of grass carp (Ctenopharyngodon idella) in aquaculture worldwide, selective breeding programs for growth traits remain limited. This study aimed to assess the genetic effects and extent of genotype by environment interactions (G × E) for early growth traits in grass carp. Fifty-eight full-sib grass carp families (n = 1704; 20 dams and(More)
Here we report the isolation and characterization of 14 polymorphic loci isolated from a partial genomic DNA library of hard shelled mussel (Mytilus coruscus) enriched for CA repeats. We tested variability of these microsatellites on 60 unrelated individuals collected in China. All loci were polymorphic. The average allele number was 19.9 per locus, ranging(More)
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