Yu. A. Trizna

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A simple and fast procedure for obtaining labeled monoclonal antibodies (mABs) from hybridoma culture fluid in amounts sufficient for selection of antibody pairs in a sandwich ELISA and for mAB affinity assessment has been developed. The procedure is based on a novel approach involving the binding of mABs present in hybridoma culture fluid to antigen(More)
Using ion chromatography and gel filtration we showed that at acidic pH values a caspase-3 substrate may be cleaved by different proteases of the brain. Previously, we showed that one of these enzymes is cathepsin B. Here, we used substrate analysis and Western blotting and found that the proteasome has the capacity to cleave a caspase-3 substrate at acidic(More)
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