Youn Min Park

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The metC gene of Salmonella typhimurium was cloned into the plasmid vectors pACYC184 and pBR322. Genetic and biochemical experiments indicate that the region controlling metC gene expression is present on the cloned fragments. The location of the metC gene was determined by insertional inactivation with transposons Tn5 and mini-Mu. The gene product was(More)
BACKGROUND Sialic-acid-binding immunoglobulin-like lectins (Siglecs) are the best-characterized immunoglobulin-type lectins. There is a growing amount of data linking Siglec and autoimmune diseases. The recently identified Siglec-9 inhibits T cell receptor (TCR)-mediated signaling which has been demonstrated by site-directed mutagenesis. In human Siglec-9,(More)
We used an Escherichia coli lac deletion strain lysogenized with a metC-lacZ fusion phage (lambda Clac) to select operator-constitutive mutations in the Salmonella typhimurium metC gene control region. The mutations were located in a region containing 2 tandemly repeated 8 bp palindromes previously proposed to be the MetJ repressor binding site. Lysogens(More)
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