Yoshiyuki Takiyama

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A pancreatic cancer cell line, PC-1, was established from a pancreatic ductal carcinoma induced in a hamster by N-nitrosobis(2-oxopropyl)amine (BOP). The cells grew in a monolayer with a doubling time of 38 h, and floated or piled up to form a duct-like structure. Chromosome counts ranged from 42 to 89. Light and electron microscopic studies of PC-1 cells(More)
To understand high malignancy of pancreatic cancer, the growth and metastatic patterns of pancreatic cancer induced in Syrian hamsters were examined. In this model, induced tumors resemble the human disease morphologically, clinically, biologically, and immunologically. In the current study, primary-induced cancer and transplants of pancreatic cancer cell(More)
Blood group A, B, H, Le, Leb, Lex, and Ley antigenicity as well as the expression of CA 19-9 were examined in pancreatic cancer specimens from 30 patients, using monoclonal antibodies to the respective antigen and immunohistochemical techniques, and the findings were correlated with the blood group types (ABO and Lewis) of the individuals. Compatible(More)
Our previous studies have shown that pancreatic cancer induced in Syrian hamsters by N-nitrosobis(2-oxopropyl)amine (BOP) shows remarkable similarities with the human disease in morphologic and biologic characteristics. Moreover, both human and hamster pancreatic cancer share expression of some tumor-associated antigens, such as those with blood group(More)
The subcellular localization and biochemical characteristics of blood group A antigen were studied by immunogold methods and by SDS-PAGE and Western blotting procedures in N-nitrosobis)2-oxopropyl)amine (BOP)-induced pancreatic cancer (PC) in Syrian hamsters, in the pancreatic cancer cell line (PC-1) derived from a primary induced pancreatic cancer, and in(More)
Expression of blood group-related antigens and the binding pattern of Ulex europaeus-I (UEA-I) in fetal and newborn hamster pancreases was examined immunohistochemically by monoclonal antibodies (MoAbs) against blood group antigens and by peroxidase-conjugated UEA-I. The fetal hamster pancreas was detected histologically at the 12th day of gestation. MoAbs(More)
The expression of PA8-15 antigen and the blood-group-related antigens A, B, O, Lea, Leb, Lex, and Ley, as well as CA19-9, were examined in the normal pancreas and in specimens from benign and malignant pancreatic tissue by the avidin-biotin-immunoperoxidase technique. A correlation was found between the expression of PA8-15, Lea, and CA19-9 in some cases.(More)
DAP12 is a recently cloned, immunoreceptor tyrosine-based activation motif-bearing transmembrane adapter molecule that is associated with the NK-activating receptors. Previous reports showed that the DAP12 message could be detected not only in NK cells but also in granulocytes, monocytes, dendritic cells, and macrophages. In this study we found a(More)
Inoculation of the pancreatic cancer cell line PC-1 into a defined area of the tail of the hamster pancreas led to development of multiple cancerous foci in the body of the pancreas along the main pancreatic duct. Each carcinoma showed identical histological characteristics and was completely separated from the others by regions of normal pancreatic tissue.(More)
The binding patterns of the lectin Ulex Europaeus-I (UEA-I) to pancreatic cells of Wistar rats from TNO, in azaserine-induced acinar cell lesions, was examined by peroxidase-conjugated UEA-I. In the normal rat, acinar cells showed this lectin binding to luminal and intracytoplasmic cell membranes. Four different types of acinar cell nodules could be(More)