Yoshimi Imada

Learn More
A gene encoding the mature Escherichia coli heat-labile enterotoxin (LT) lacking the nick site in the A subunit by deleting tripeptides was introduced in a vector pNH301 and expressed extracellularly as mutant molecule of holotoxin at high levels in Bacilus brevis HPD31-S5 of the host bacterium. The mucosal adjuvant activities of the produced mutant LT(More)
In adult C57BL/6 mice, a high-responder strain to ovalbumin, PCA and HA antibodies appeared at the same time after immunization. In young mice, on the other hand, the capacity to produce IgE antibodies against ovalbumin developed earlier than that to produce IgM or IgG antibodies.
Placental growth factor-2 (PlGF-2) exhibits neurotrophic activity in dorsal root ganglion (DRG) neurons through the neuropilin-1 (NP-1) receptor in vitro. To examine the potential utility of PlGF-2 therapy for treating diabetic neuropathy, we performed intramuscular PlGF-2 gene transfer by electroporation, and examined its effects on sensory neuropathy in(More)
The effect of nutrients of natural source, such as corn steep liquor, peptone, and yeast extract, on the fermentative production of L-glutamic acid from hydrocarbons by a Corynebacterium was studied. Corn steep liquor and meat extract were found to be remarkably stimulatory to L-glutamic acid production; about 5 g per liter of L-glutamic acid were(More)
Mice were subjected to thymectomy at various times after birth and immunized with ovalbumin in aluminum hydroxide gel at 4 or 8 weeks of age. The capacities to produce IgE, IgM and IgG antibodies were abolished by thymectomy carried out within 48 h after birth. The capacity to produce IgE antibody was not affected by thymectomy at 7 days or later, whereas(More)
In the central nervous system, ZM decreased locomotor activity and potentiated hypnosis of hexobarbital-Na in mice. ZM had little hypothermic action and there were no anticonvulsive effects on chemoconvulsion and electroconvulsion shock. ZM, 3 mg/kg i.v. produced a sleep-like pattern in the spontaneous EEG activity of cat; from 20 to 30 min. after(More)