Yoshimi Ikemoto

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BACKGROUND The molecular mechanism of propofol anesthesia has been related to facilitation of the inhibitory neurotransmission mediated by gamma-aminobutyric acid (GABA). In the current study, the authors examined the direct actions of propofol on the acutely dissociated mammalian central neurons. METHODS Hippocampal pyramidal neurons were dissociated(More)
The pharmacological properties of the L-glutamate (Glu)-induced chloride current (ICl) in enzymatically isolated Aplysia neurones were examined using the 'concentration clamp' technique. The Glu-ICl did not cross-desensitize with the ICl evoked by gamma-aminobutyric acid or acetylcholine. Quisqualate, kainate (one out of eight) and N-methyl-D-aspartate (one(More)
The effects of gamma-aminobutyric acid (GABA) on the glutamate receptor chloride ion (Cl-) channel complex were examined in mechanically isolated and internally perfused Aplysia neurons using a concentration clamp technique. GABA at concentrations of 3 x 10(-6) M or more, concentration dependently delayed the recovery of the glutamate response from(More)
1. Effects of two volatile anesthetics [halothane (Hal) and enflurane (Enf)] and a volatile convulsant [hexafluorodiethyl ether (HFE)] on amino acid-induced membrane currents in neurons dissociated from the nucleus tractus solitarius of the rat were examined. The dissociated neurons were voltage clamped in the whole-cell mode of the patch-clamp technique.(More)
(1) Kinetics of activation and desensitization phases of the ACh-induced chloride current (I Cl) were studied in isolated single neurones ofAplysia kurodai, using the ‘concentration clamp’ technique which combines internal perfusion and rapid exchange of the external solution within a few milliseconds (2) The dose-response curve for the peakI Cl gave a(More)
Effects of two general anesthetics, halothane and thiamylal, on the fast sodium inward current (INa) of enzymatically isolated single rat ventricular cells were studied under current clamp and voltage clamp conditions. A suction pipette technique was used for potential measurement, current injection and internal perfusion of isolated cells. In current clamp(More)
The effect of pentobarbital sodium (PB) on the gamma-aminobutyric acid (GABA)-induced macroscopic and microscopic Cl- currents (ICl and iCl, respectively) was studied in the soma membrane of isolated frog sensory neurons using a rapid concentration-jump and patch-clamp technique. The GABA-induced ICl was composed of a transient peak and a steady plateau. PB(More)
BACKGROUND Activation of the gamma-aminobutyric acidA (GABAA) receptor-ionophore complex has been reported as a possible molecular mechanism of the anesthetic action of propofol. Augmentation of GABA-induced inhibitory transmission has also been suggested as a mechanism. Because data describing this latter mechanism in mammalian neurons are few, we have(More)
L-Glutamate (Glu) applied by the 'concentration clamp' technique to isolated neurones of Aplysia induced a chloride current (ICl) by activating a single population of the channel. The concentration-response curve for the peak ICl gave a dissociation constant of 1.3 x 10(-4) M and a Hill coefficient of 1.8. The current-voltage relationship was linear in the(More)
The effects of tetrodotoxin and lidocaine on the voltage-dependent sodium current (INa) were studied in the CA1 pyramidal neurons isolated acutely from rat hippocampus using a 'concentration-clamp' technique which combines the intracellular perfusion with a rapid external solution change within a few ms. Tetrodotoxin (TTX) exerted its inhibitory action in(More)