Yoshimasa Saburi

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From the genomic library of Pseudomonas paucimobilis TMY1009 constructed with the cosmid pHC79, an 8-kb BamHI-KpnI fragment encoding lignostilbene-alpha,beta-dioxygenase (LSD) was cloned into pUC19 (designated pKSN2510). E. coli JM109 having pKSN2510 produced a small amount of LSD only when the lac promoter was induced by(More)
Three isozymes of lignostilbene-alpha,beta-dioxygenase (LSD) from Pseudomonas paucimobilis TMY1009 were separated on QAE-Toyopearl chromatography. All active fractions were further chromatographed on DEAE-Toyopearl, Butyl-Toyopearl, and Sephacryl S-300 columns. Then the isozymes I, II, and III were purified homogeneously. All three isozymes consisted of two(More)
Lignostilbene-alpha, beta-dioxygenase isozyme IV was purified from ultrasonic extracts of Pseudomonas paucimobilis TMY1009 through four steps of column chromatography. The fraction obtained gave a single band on SDS-PAGE and a single peak on reversed-phase HPLC and DEAE-HPLC. The specific activity and Km of purified isozyme IV were 110 mu kat/g and 4.2(More)
Allergic rhinitis caused by pollen of Japanese cedar (Cryptomeria japonica) is found in Japan. These pollens, when inhaled into the nasal cavity, contact the nasal mucus membrane, and the allergens separate from the pollens, and pass through the nasal mucosa to interact with the mast cell-bound IgE. Patients with allergic rhinitis produce a great volume of(More)
A genomic DNA library of Pseudomonas paucimobilis TMY1009 was constructed using a cosmid vector pWE15. Screening of the library for lignostilbene-alpha,beta-dioxygenase (LSD) isozyme genes was done with a common probe for the alpha, beta, and gamma subunits, which composed LSD isozymes. The positive clones obtained by colony hybridization were further(More)
A common structure of substrates of lignostilbenedioxygenases was investigated using synthesized stilbenes. Cell-free extracts of Sphingomonas paucimobilis TMY1009 degraded only trans-4-hydroxystilbene and trans-4-hydroxy-3-methoxystilbene. Other stilbenes that had no 4-hydroxyl group and had a cis structure were not substrates for(More)
A reciprocal translocation involving the short arms of chromosomes 7 and 11, t(7;11)(p15;p15), was found in nine patients including eight with acute myelogenous leukemia (AML) and one with Philadelphia (Ph1) chromosome-positive chronic myelogenous leukemia (CML) in blastic crisis. Although a similar chromosome rearrangement has previously been reported in(More)
Lignostilbene-alpha,beta-dioxygenase (LSD, EC is involved in oxidative cleavage of the central double bond of lignostilbene to form the corresponding aldehydes by a mechanism similar to those of 9-cis-epoxycarotenoid dioxygenase and beta-carotene 15,15'-dioxygenase, key enzymes in abscisic acid biosynthesis and vitamin A biosynthesis,(More)
The contents of diterpenoids, ginkgolides A, B, and C, in seeds, embryos, and plantlets ofGinkgo biloba were analyzed to clarify the relations between organogenesis and terpene contents inG. biloba. There is so far no published report on the contents and changes in such terpenes in seeds and very young plantlets ofG. biloba. Ginkgolides were present in(More)
The stilbene glucoside isorhapontin (5,4′-dihydroxy-3′-methoxystilbene-3-β-D-glucoside) is the major constituent of the ethyl acetate extracts fromPicea glehnii bark. Isorhapontin inhibited the hydrolytic activity ofTrichoderma cellobiohydrolase I (CBH I) for both bacterial microcrystalline cellulose and the soluble cellooligosaccharide celloheptaitol. The(More)