Yoshikazu Shimomura

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PURPOSE To evaluate the preventive effect of vaccination using fusion protein (gD-IL-2) consisting of herpes simplex virus type 1 (HSV-1) glycoprotein D (gD), and human interleukin-2 (IL-2), and plasmid DNA encoding gD-IL-2 against murine herpetic keratitis. METHODS Plasmid containing gD-IL-2 (pHDLneo1) was constructed, and gD-IL-2 peptide was purified.(More)
PURPOSE To quantify the degree of metamorphopsia in patients with idiopathic epiretinal membranes (ERMs), by use of a new metamorphopsia chart (M-CHARTS) developed by the authors. METHODS These M-CHARTS had 19 dotted lines with dot intervals of between 0.2 degrees (fine) and 2.0 degrees (coarse) visual angles. As the dot intervals were changed from fine(More)
PURPOSE Methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant coagulase-negative Staphylococcus (MR-CNS) are two major multidrug-resistant pathogens. In this paper we report the prevalence of MRSA and MR-CNS in ocular surface infections. METHODS We investigated the proportion of MRSA and MR-CNS in bacterial isolates from conjunctiva(More)
Ocular HSV-1 shedding from latently infected rabbits was induced by iontophoresis of 0.01% epinephrine into the eye. Anodal Iontophoresis of epinephrine was performed at 0.8 mAmps for 8 min once a day for 3 consecutive days. Shedding was determined by the presence of HSV-1 in the preocular tear film obtained via eye swabs. Bilateral epinephrine(More)
Iontophoresis of 0.01% levo(-) epinephrine for 8 min at 0.8 mAmp once daily for 3 consecutive days induces ocular shedding of herpes simplex virus type 1 (HSV-1) in latently infected rabbits. In the present experiment, we tested dextro(+) and levo(-) epinephrine for their comparative effects on induced HSV-1 ocular shedding. One hundred percent of the eyes(More)
To record electroretinograms (ERG) produced by short-wavelength-sensitive cone mechanisms (SWS-cone ERG), the authors used three kinds (blue, green, and red) of light-emitting diode (LED) which were built into a contact lens electrode assembly. The LEDs were used as both stimulus and background light sources. ERG was recorded using blue LED after 10 min of(More)
PURPOSE To review our previous studies regarding alterations in gene expression in HSV-1 latently infected mouse trigeminal ganglia (TGs) following treatment with immunosuppressants and hyperthermia. METHODS Uninfected and HSV-1 latently infected mice were treated with immunosuppressants or heat stressed (43 degrees C for 10 minutes). In the(More)
PURPOSE The purpose of the study was to investigate the recurrence-free interval after phototherapeutic keratectomy (PTK) in patients with corneal dystrophies resulting from an Arg124His (R124H) mutation of the Big-h3 gene. METHODS Patients with corneal dystrophy resulting from a genetically confirmed Big-h3 R124H mutation were examined with a slit lamp.(More)
The development of an animal model for herpes simplex virus (HSV) reactivation is of great importance in studying HSV latency, reactivation, recurrence, and chemotherapy. Epinephrine iontophoresis to the cornea can induce HSV type 1 (HSV-1) ocular shedding from latently infected rabbits. In the present experiment, adrenergic induction of HSV-1 was enhanced(More)
PURPOSE To detect herpes simplex virus (HSV) genome in the cornea, we sampled the limbal corneas and scleras of the imported eye bank eyes and recipient's corneal buttons and quantitated HSV genome in them by real-time polymerase chain reaction (PCR). METHODS Forty-four recipient corneas including 7 corneas with and 37 corneas without a history of(More)