• Publications
  • Influence
The crystal structure of spermidine synthase with a multisubstrate adduct inhibitor
TLDR
The crystal structure of the PAPT from Thermotoga maritima has been solved to 1.5 Å resolution and reveals deep cavities for binding substrate and cofactor, and a loop that envelops the active site, suggesting a universal catalytic mechanism.
Aminopropyltransferases: function, structure and genetics.
TLDR
A general mechanism for catalysis by aminopropyltransferases can be proposed based on studies and key regions in the active site were identified by structural studies with spermidine synthase from Thermotoga maritima bound to S-adenosyl-1,8-diamino-3-thiooctane, a multisubstrate analog inhibitor.
Structure and mechanism of spermidine synthases.
TLDR
Comparison of the structures of the human spermidine synthase with both substrates and products and analysis of the properties of site-directed mutants provide a general mechanistic hypothesis for the aminopropyl transfer reaction.
Determination of Organic Phosphates by Column-Switching High Performance Anion-Exchange Chromatography Using On-Line Preconcentration on Titania
A highly selective organic phosphates analyzer was developed based on a column-switching technique using a titania (TiO2) precolumn and an anion-exchange analytical column. In the preliminary
Crystal Structure of Human Spermine Synthase
TLDR
The spermine synthase·5′-methylthioadenosine structure provides a plausible explanation for the potent inhibition of the reaction by this product and the stronger inhibition of spermining synthase compared with spermidine synthase.
Spermine Synthesis Is Required for Normal Viability, Growth, and Fertility in the Mouse*
TLDR
It is shown that spermine synthesis is needed for normal growth, viability, and fertility in male mice and that regulation of sPermine synthase content is not required.
X-linked spermine synthase gene (SMS) defect: the first polyamine deficiency syndrome
TLDR
The clinical features observed in SRS are consistent with cerebellar dysfunction and a defective functioning of red nucleus neurons, which, at least in rats, contain high levels of spermine, and the presence of MR reflects a role for s permine in cognitive function, possibly by sper mine's ability to function as an ‘intrinsic gateway’ molecule for inward rectifier K+ channels.
Rational Design of Small-Molecule Stabilizers of Spermine Synthase Dimer by Virtual Screening and Free Energy-Based Approach
TLDR
It is suggested that druggable pockets exist in the vicinity of the mutation sites at protein-protein interfaces which can be used to alter the disease-causing effects by small molecule binding.
Characterization of transgenic mice with widespread overexpression of spermine synthase.
TLDR
Results provide strong support for the concept that the levels of the higher polyamines spermidine and spermine are not determined only by the relative activities of the two aminopropyltransferases.
Brain infarction correlates more closely with acrolein than with reactive oxygen species.
TLDR
The relative importance of acrolein and ROS in cell damage during brain infarction was compared using photochemically induced thrombosis model mice and it was shown thatinfarction led more readily to RNA damage than to DNA or phospholipid damage.
...
1
2
3
...