Yoshifusa Sadamura

Publications8
h-index3
Citations263
Highly Influential Citations7
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A novel affinity-based method for the isolation of highly purified extracellular vesicles
TLDR
Tim4 purification, which is applied to cell conditioned media and biofluids, is capable of yielding EVs of a higher purity than those obtained using conventional methods, and allows more EV-specific proteins to be detected by mass spectrometry, enabling better characterization and quantification of different EV populations’ proteomes.
Claudin-5-Binders Enhance Permeation of Solutes across the Blood-Brain Barrier in a Mammalian Model
TLDR
In an in vitro model of the blood-brain barrier, the anti–CLDN-5 mAbs attenuated trans-epithelial/endothelial electrical resistance and enhanced solute permeation are potential leads for the development of novel drug delivery systems targeting the CNS.
Development of a practical sandwich assay to detect human pluripotent stem cells using cell culture media
Visualizing looping of two endogenous genomic loci using synthetic zinc-finger proteins with anti-FLAG and anti-HA frankenbodies in living cells
TLDR
The ability to image two endogenous genomic loci simultaneously in single living cells provides a proof of principle that ZF probes coupled with frankenbodies are useful new tools for exploring genome dynamics in multiple colors.
Forkhead box B2 inhibits the malignant characteristics of the pancreatic cancer cell line Panc‐1 in vitro
TLDR
FoxB2 has the ability to inhibit the malignant characteristics of Panc‐1 in vitro, and this work suggests that it acts as a tumor suppressor.
Application of PS-affinity-based methods to extracellular vesicle research
Claudin‐5 as a target for drug delivery to the brain by modulation blood‐brain barrier
Visualizing looping of two endogenous genomic loci using synthetic zinc-finger proteins with anti-FLAG and anti-HA frankenbodies in living cells
TLDR
The ability to image two endogenous genomic loci simultaneously in single living cells provides a proof-of-principle that ZF probes coupled with frankenbodies are useful new tools for exploring genome dynamics in multiple colors.