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OBJECTIVE The expression of DACH1 was frequently lost in human breast cancer, which significantly correlated with poor prognosis. Herein, we aim to investigate its underlying mechanisms. METHODS The expression of miR-217 was detected by Taqman PCR. The mRNA and protein level of DACH1 were investigated by real time PCR and western blot. The dual-luciferase(More)
The mobility of proteins adsorbed on a solid substrate and on a lipid monolayer was measured by fluorescence recovery after photobleaching. Both a conventional fluorescence microscope with a charge-coupled device camera and a laser scanning confocal microscope were used. For proteins adsorbed on a solid substrate, the bleached area never recovered fully,(More)
BACKGROUND Peripheral artery disease (PAD), which is caused by atherosclerosis, results in progressive narrowing and occlusion of the peripheral arteries and inhibits blood flow to the lower extremities. Therapeutic angiogenesis is a promising strategy for treating ischemia caused by PAD. Nitric oxide (NO) has been shown to be a key mediator of(More)
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