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γ-Aminobutyric acid (GABA), a non-protein amino acid, is a bioactive component in the food, feed and pharmaceutical fields. To establish an effective single-step production system for GABA, a recombinant Corynebacterium glutamicum strain co-expressing two glutamate decarboxylase (GAD) genes (gadB1 and gadB2) derived from Lactobacillus brevis Lb85 was(More)
To understand the molecular mechanisms of development it is essential to be able to turn genes on and off at will and in a spatially restricted fashion. Morpholino oligonucleotides (MOs) are very common tools used in several model organisms with which it is possible to block gene expression. Recently developed photo-activated MOs allow control over the(More)
A feedback between decomposition and litter chemical composition occurs with decomposition altering composition that in turn influences the decomposition rate. Elucidating the temporal pattern of chemical composition is vital to understand this feedback, but the effects of plant species and climate on chemical changes remain poorly understood, especially(More)
The impact of understory vegetation control or replacement with selected plant species, which are common forest plantation management practices, on soil C pool and greenhouse gas (GHG, including CO2, CH4 and N2O) emissions are poorly understood. The objective of this paper was to investigate the effects of understory vegetation management on the dynamics of(More)
To investigate the mode of action of leucocin K7 against Listeria monocytogenes and to assess its inhibitory effect on Lis. monocytogenes in refrigerated milk. A bacteriocin-producing strain, Leuconostoc mesenteroides K7, was isolated from a fermented pickle. The bacteriocin, leucocin K7, exhibited antagonistic activity against Lis. monocytogenes with an(More)
Deoxynivalenol (DON) is a secondary metabolite produced by Fusarium fungi, which is found in a wide range of agricultural products, especially in wheat, barley, oat and corn. In this study, the distribution of DON in the wheat kernel and the effect of exposure time to ozone on DON detoxification were investigated. A high concentration of toxin was found in(More)
Corynebacterium glutamicum ssp. lactofermentum strain JHI3-156 produces l-isoleucine (Ile). Overexpression of the Saccharomyces cerevisiae-derived NADH kinase gene (POS5) and the endogenous glucose-6-phosphate dehydrogenase and NAD kinase genes (zwf-ppnK) in JHI3-156 increased Ile production by 26 and 31 %, respectively. To decipher the global effect of(More)
To improve the effectiveness and robustness of fatigue driving recognition, a self-adaptive dynamic recognition model is proposed that incorporates information from multiple sources and involves two sequential levels of fusion, constructed at the feature level and the decision level. Compared with existing models, the proposed model introduces a dynamic(More)