Yong-chun Xu

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OBJECTIVE To determine whether polymorphisms in interleukin-1B gene promoter -31 and exon 5 +3953 loci are associated with coronary heart diseases (CHD) in Chengdu Han population. METHODS Two SNPs of IL-1B gene (+3953C/T and -31T/C) in 100 patients with CHD (CHD group) and 144 healthy controls in Chengdu were analysed using polymerase chain(More)
OBJECTIVE To prepare a monoclonal antibody (mAb) against GST-Rta150 fusion protein from EB virus and identify its characteristics. METHODS BALB/c mouse was rendered immune by GST-Rta150 fusion protein; after that, the spleen cells of immunized mouse were taken to fuse with SP2/0 myeloma cells by a routine method. Then screening for positive hybridoma(More)
Binding interactions and Raman spectra of water in hydrogen-bonded anionic complexes have been studied by using the hybrid density functional theory method (B3LYP) and ab initio (MP2) method. In order to explore the influence of hydrogen bond interactions and the anionic effect on the Raman intensities of water, model complexes, such as the negatively(More)
OBJECTIVE To obtain the recombinant fusion proteins GST-Rta185 and GST-Rta150 from EB virus and prepare two Rta protein specific polyclonal antibodies, respectively. METHODS Plasmids pGEX-R1501, pGEX-R1851 and pGEX-5X-3 were separately transformed into Escherichia coli BL21 (DE3). Expressions of the recombinant proteins R150-GST, R185-GST and free GST(More)
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