Yong Wang

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Quantum dots are promising candidates for single molecule imaging due to their exceptional photophysical properties, including their intense brightness and resistance to photobleaching. They are also notorious for their blinking. Here we report a novel way to take advantage of quantum dot blinking to develop an imaging technique in three-dimensions with(More)
The mechanosensitive channel of large conductance, which serves as a model system for mechanosensitive channels, has previously been crystallized in the closed form, but not in the open form. Ensemble measurements and electrophysiological sieving experiments show that the open-diameter of the channel pore is >25 Å, but the exact size and whether the(More)
We developed a coating method to produce functionalized small quantum dots (sQDs), about 9 nm in diameter, that were stable for over a month. We made sQDs in four emission wavelengths, from 527 to 655 nm and with different functional groups. AMPA receptors on live neurons were labeled with sQDs and postsynaptic density proteins were visualized with(More)
Immunofluorescence, a powerful technique to detect specific targets using fluorescently labeled antibodies, has been widely used in both scientific research and clinical diagnostics. The probes should be made with small antibodies and high brightness. We conjugated GFP binding protein (GBP) nanobodies, small single-chain antibodies from llamas, with new ∼7(More)
Please address questions to press@elifesciences.org. Details on how to cite eLife articles in news stories and our media policy are available at The mechanosensitive channel of large conductance, which serves as a model system for mechanosensitive channels, has previously been crystallized in the closed form, but not in the open form. Ensemble measurements(More)
Determining the structures, kinetics, thermodynamics and mechanisms that underlie conformational exchange processes in proteins remains extremely difficult. Only in favourable cases is it possible to provide atomic-level descriptions of sparsely populated and transiently formed alternative conformations. Here we benchmark the ability of enhanced-sampling(More)
This study explores the association between intergenerational relationships and life satisfaction among urban elders in China, with a focus on the emotional dimension of intergenerational solidarity. Individual factors, effects of associational, affectual, functional and structural solidarity were examined by analyzing data collected from the Zhenjiang City(More)
In neurons, microtubule motor driven transport is crucial for communication between processes and the cell body. Disruptions in transport are associated with a variety of neurodegenerative diseases. Recent studies implicate phosphorylation of serine 175, a conserved residue found in all three isoforms of kinesin-1 in impaired axonal transport associated(More)
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