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Interferen-γ of Rongchang porcine was amplifed by RT-PCR with the total RNA extracted from the peripheral blood lymphocytes. The gene was sequenced after cloned into the vector pMD18-T and analyzed with molecular biological software. The results showed that the opening reading frame (ORF) of interferen-γ of Rongchang porcine consists of 501(More)
D-Pantonohydrolase has attracted increasing attention as a biocatalyst for stereospecific production of D-pantoic acid. The Fusarium moniliforme D-pantonohydrolase was selected for directed evolution through error-prone Polymerase Chain Reaction (PCR) combined with DNA shuffling for improved activity and pH stability using a convenient two-step(More)
D-lactonohydrolase is useful in the procedure of resolution of racemic pantolactone to produce D-pantolactone, but the activity and stability under low pH of the wild type enzyme is not satisfactory enough to be applied to industrial production. The expected properties of wild type enzyme were enhanced by directed evolution. According to the formation of(More)
Porcine transmissble gastroenteritis (TGE) is a highly contagious enteric disease of swine caused by transmissible gastroenteritis virus (TGEV). The disease is especislly severe in piglets under two weeks of age, characterized by vomiting, diarrhea and nearly 100% mortality. In this study, the complete genome sequence is available from SC-Y strain isolated(More)
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