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Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis
The genome sequence provides insight into how P. stipitis regulates its redox balance while very efficiently fermenting xylose under microaerobic conditions.
Engineered Saccharomyces cerevisiae capable of simultaneous cellobiose and xylose fermentation
Improved yields and productivities from cofermentation experiments performed with simulated cellulosic hydrolyzates are observed, suggesting this is a promising coferment strategy for cellulosIC biofuel production.
Saccharomyces cerevisiae Engineered for Xylose Metabolism Exhibits a Respiratory Response
The results suggest that recombinant S. cerevisiae does not recognize xylose as a fermentable carbon source and that respiratory proteins are induced in response to cytosolic redox imbalance; however, lower sugar uptake and growth rates on xylOSE might also induce transcripts for respiration.
Optimal Growth and Ethanol Production from Xylose by Recombinant Saccharomyces cerevisiae Require Moderate d-Xylulokinase Activity
To bypass the effect of overexpression of genes for XK, cells were transformed with a tunable expression vector containing XYL3 under the control of its native promoter into the FPL-YS1020 strain and screened the transformants for growth on, and ethanol production from, xylose.
Maternal fucosyltransferase 2 status affects the gut bifidobacterial communities of breastfed infants
Mechanistic insight is provided into how milk glycans enrich specific beneficial bacterial populations in infants and clues for enhancing enrichment of bifidobacterial populations in at risk populations - such as premature infants are revealed.
Bacterial Genome Editing with CRISPR-Cas9: Deletion, Integration, Single Nucleotide Modification, and Desirable "Clean" Mutant Selection in Clostridium beijerinckii as an Example.
By combining inducible expression of Cas9 and plasmid-borne editing templates, this study successfully achieved gene deletion and integration with high efficiency in single steps and employed CRISPR-Cas9 as an efficient tool for selecting desirable "clean" mutants in this study.
Rational and Evolutionary Engineering Approaches Uncover a Small Set of Genetic Changes Efficient for Rapid Xylose Fermentation in Saccharomyces cerevisiae
Deletion of ALD6 coding for acetaldehyde dehydrogenase not only prevented acetate accumulation, but also enabled complete and efficient fermentation of xylose as well as a mixture of glucose andxylose by the evolved strain, providing direct guidance for developing industrial strains to produce cellulosic fuels and chemicals.