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We report measurements of the incorporation of radioactive molecules during short labeling periods, as a function of cell-cycle stage, using a cell-sorter-based technique that does not require cell synchronization. We have determined: (1) tritiated thymidine (3H-TdR) incorporation throughout S-phase in Lewis lung tumor cells in vitro both before and after… (More)
This paper describes a flow-cytometric application of the quenching of fluorescence from 33258 Hoechst stained Chinese hamster ovary-line cells due to the incorporation of 5-bromo-deoxyuridine (BrdU) into the cellular deoxyribonucleic acid. Cells were grown for 24 hr in medium containing BrdU in concentrations ranging from 1 x 10(-8) to 1 x 10(-4) M. For… (More)
OIA teer services to public schools, museums , public interest groups, hospitals and comnmnity service organizations. Others receive help and share ideas about financial services, including financial planning, money management and tax preparation. On-line issues, forums and special interest groups permit members to access information about health issues,… (More)
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deputy director and program director, Directorate of Education and Human Resources, provided names of potential participants, served on the advisory committee, and made major contributions to the program. In addition, AAAS provided space for planning meetings and co-hosted the conference at its headquarters in Washington, DC. We also want to express our… (More)
In this paper, we present a procedure for the rapid, quantitative estimation of the G1, S, and G2 + M phase durations and dispersions and the growth fraction for asynchronously growing cell populations. In this procedure, the cell population is pulse-labelled with a radioactive DNA precursor at the beginning of the analysis and then sampled periodically.… (More)
A new rapid method for the cell cycle analysis of asynchronously growing cells is presented. The new method is an alternative to the more time consuming and subjective fraction of labeled mitoses (FLM) method. Like the FLM method, all cells in the S phase of the cell cycle are marked by pulse labeling with a radioactive DNA precursor. The subsequent… (More)