Yolanda J Brady

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Limited information is available on digestibility of nutrients in various practical ingredients used in diets for commercially important finfish species, such as hybrid striped bass. This information is especially needed for sunshine bass, Morone chrysops 3 M. saxatilis, to improve least-cost diet formulations and to allow effective substitution of(More)
Chemokines are a family of structurally related chemotactic cytokines that regulate the migration of leukocytes, under both physiological and inflammatory conditions. CC chemokines represent the largest subfamily of chemokines with 28 genes in mammals. Sequence conservation of chemokines between teleost fish and higher vertebrates is low and duplication and(More)
Golden shiners (Notemigonus crysoleucas) experimentally infected with four reoviruses supported replication of golden shiner virus as well as chum salmon virus, reovirus 13p2 and catfish reovirus at temperatures of 23 and 28 C. All four reoviruses replicated in golden shiners in this study. Natural infections of the golden shiner virus are known only in(More)
BACKGROUND EST sequencing is one of the most efficient means for gene discovery and molecular marker development, and can be additionally utilized in both comparative genome analysis and evaluation of gene duplications. While much progress has been made in catfish genomics, large-scale EST resources have been lacking. The objectives of this project were to(More)
The 1035 base-pair (bp) open reading frame 59 (ORF59) of channel catfish virus (CCV) codes for an abundant hydrophobic membrane glycoprotein considered to be the major envelope glycoprotein of the virus. The ORF59 gene of CCV was cloned into the baculovirus genome by site-specific transposition and expressed in Spodoptera frugiperda cells (Sf9 cells).(More)
Shellstock refrigeration after harvesting is recommended to prevent further increases in Vibrio vulnificus numbers in oysters, but it could potentially induce a cold shock response in this bacterium. V. vulnificus was incubated at 35, 25, 20, and 15 degrees C and then subjected to 7.2 and 4 degrees C for 1 week. A cold-adaptation response that enhanced cell(More)
AIMS To determine Vibrio vulnificus response to shellstock refrigeration conditions while the bacterium was embedded in oysters (in vivo). METHODS AND RESULTS Depurated oysters were artificially inoculated with V. vulnificus. Several cold-shock conditions were examined according to the National Sanitation Shellfish Program guidelines. Culturability of(More)
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