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Production of L-malic acid with fixation of HCO3(-) by malic enzyme-catalyzed reaction based on regeneration of coenzyme on electrode modified by layer-by-layer self-assembly method.
- H. Zheng, Yoko Ohno, T. Nakamori, S. Suye
- Medicine, Chemistry
- Journal of bioscience and bioengineering
Malic enzyme prepared and purified from Brevundimonas diminuta IFO13182 catalyzed the decarboxylation reaction of malate to pyruvate and CO2 using NAD+ as the coenzyme, and the reverse reaction was… Expand
Reverse Reaction of Malic Enzyme for HCO3 − Fixation into Pyruvic Acid to Synthesize L-Malic Acid with Enzymatic Coenzyme Regeneration
- Yoko Ohno, T. Nakamori, H. Zheng, S. Suye
- Biology, Medicine
- Bioscience, biotechnology, and biochemistry
- 23 May 2008
Malic enzyme [L-malate: NAD(P)+ oxidoreductase (EC 18.104.22.168)] catalyzes the oxidative decarboxylation of L-malic acid to produce pyruvic acid using the oxidized form of NAD(P) (NAD(P)+). We used a… Expand
Coimmobilization of malic enzyme and alanine dehydrogenase on organic–inorganic hybrid gel fibers and the production of L‐alanine from malic acid using the fibers with coenzyme regeneration
Malic enzyme (EC 22.214.171.124) and alanine dehydrogenase (EC 126.96.36.199) were entrap-immobilized on hybrid gel fibers of cellulose acetate (CA) and zirconium (Zr) alkoxide by air-gap wet spinning. The… Expand