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Overwintering larvae of the rice stem borer, Chilo suppressalis accumulate glycerol and are freezing tolerant to about -25 degrees C. However, non-diapausing larvae cannot accumulate glycerol and are killed by freezing. We compared the extent of tissue damage, the effects of glycerol concentration, and the transport of glycerol and water in fat body tissues(More)
Cold hardiness of the freshwater apple snail, Pomacea canaliculata, varies seasonally. We investigated lethal factors and physiological changes arising from exposure of P. canaliculata to low temperatures. Snails did not survive freezing. The supercooling point of cold-acclimated (cold tolerant) snails (-6.6+/-0.8 degrees C) did not differ significantly(More)
The complete cDNA sequences of heat shock protein 90 (hsp90) and of heat shock cognate protein 70 (hsc70) were cloned by reverse transcription polymerase chain reaction from the rice stem borer, Chilo suppressalis Walker. They potentially encode a 717-amino-acids (hsp90) and a 652-amino-acids (hsc70) protein, with calculated molecular weight of 82.5 and(More)
Environmental stresses lower the efficiency of photosynthesis and sometimes cause irreversible damage to plant functions. When spinach thylakoids and Photosystem II membranes were illuminated with excessive visible light (100-1,000 µmol photons m(-1) s(-1)) for 10 min at either 20°C or 30°C, the optimum quantum yield of Photosystem II decreased as the light(More)
The apple snail, Pomacea canaliculata, is an invasive freshwater snail. It increases its cold hardiness before winter. However, the physiological mechanism of cold hardiness in molluscs is poorly understood, especially in freshwater molluscs. In this study, we examined the changes in low molecular weight compounds, glycogen and lipids, in the body of P.(More)
To examine the effects of pesticides on insect biodiversity, a population survey was conducted in conventionally managed, low-input, and organic peach orchards. Pitfall traps were used to sample a total of 6489 insects representing 151 species at seven study sites. Results of a population survey suggest that pesticide application adversely affected insect(More)
Cicadulina bipunctata was originally distributed in tropical and subtropical regions of the Old World. This leafhopper recently expanded its distribution area to southern parts of temperate Japan. In this study, factors affecting the overwintering ability of C. bipunctata were examined. A series of laboratory experiments revealed that cold acclimation at(More)
In this study, we attempted a population survey of spiders in peach orchards with and without pesticide application; however, we had difficulty discriminating them. To identify spiders, we determined the nucleotide sequences of a ca. 640-bp region in the mitochondrial cytochrome oxidase subunit I (COI) gene from 17 species belonging to eight families. To(More)
Overwintering freeze-tolerant larvae of Chilo suppressalis can survive at -25 degrees C, but non-diapausing larvae cannot. We reported earlier that to prevent intracellular freezing, which causes death in overwintering larvae of the Saigoku ecotype distributed in southwestern Japan, water leaves and glycerol enters fat body cells through water channels(More)
Rice fields provide major habitats for lentic aquatic insects including the giant water bug Kirkaldyia (=Lethocerus) deyrolli (Heteroptera: Belostomatidae) in Japan. Previous researchers have emphasized that conserving populations of the frogs, Hyla japonicus and Rana nigromaculata, is very important for preserving K. deyrolli because these frogs were found(More)