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DNA barcoding of land plants has relied traditionally on a small number of markers from the plastid genome. In contrast, low-copy nuclear genes have received little attention as DNA barcodes because of the absence of universal primers for PCR amplification. From pooled-species 454 transcriptome data we identified two variable intron-less nuclear loci for(More)
UNLABELLED PREMISE OF THE STUDY Primers were developed to amplify 12 intron-less, low-copy nuclear genes in the Hawaiian genus Clermontia (Campanulaceae), a suspected tetraploid. • METHODS AND RESULTS Data from a pooled 454 titanium run of the partial transcriptomes of seven Clermontia species were used to identify the loci of interest. Most loci were(More)
While an excess of metals such as zinc, cadmium or nickel (Ni) is toxic for most plants, about 500 plant species called hyperaccumulators are able to accumulate high amounts of these metals. These plants and the underlying mechanisms are receiving an increasing interest because of their potential use in sustainable biotechnologies such as biofortification,(More)
UNLABELLED PREMISE OF THE STUDY Primers were developed to amplify low-copy nuclear genes in Hawaiian Metrosideros (Myrtaceae). • METHODS AND RESULTS Data from a pooled 454 Titanium run of the partial transcriptomes of four Metrosideros taxa were used to identify the loci of interest. Ten exon-primed intron-crossing (EPIC) markers were amplified and(More)
Molecular phylogenetic and morphological data show that two species of Rhamnaceae endemic to New Caledonia, Alphitonia xerocarpa and A. erubescens, are misplaced. They form a clade that is sister to Emmenosperma, and they differ from Alphitonia s.s. in their indumentum, venation, lower leaf surface, flowers, fruits and seeds. Although morphologically closer(More)
Cunoniaceae are represented in New Caledonia by some 90 species, all of them endemic, and the family is thus a significant element in this diverse island flora. We present a descriptive survey of floral biology for the seven genera present, covering floral structure and colour, inflorescence shape, sexual system and phenology, plus details of floral(More)
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