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Recently, three-dimensional reconstruction of ultrastructure of the brain has been realized with minimal effort by using scanning electron microscopy (SEM) combined with focused ion beam (FIB) milling (FIB-SEM). Application of immunohistochemical staining in electron microscopy (EM) provides a great advantage in that molecules of interest are specifically(More)
Ten years have passed since a serial block-face scanning electron microscopy (SBF-SEM) method was developed [1]. In this innovative method, samples were automatically sectioned with an ultramicrotome placed inside a scanning electron microscope column, and the block surfaces were imaged one after another by SEM to capture back-scattered electrons. The(More)
The maximum likelihood method is considered as one of the most reliable methods for phylogenetic tree inference. However, as the number of species increases, the approach quickly loses its applicability due to explosive exponential number of trees that need to be considered. An earlier work by one of the authors [3] demonstrated that, by decomposing the(More)
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