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How Does Plant Cell Wall Nanoscale Architecture Correlate with Enzymatic Digestibility?
TLDR
It is demonstrated that the small, noncomplexed fungal cellulases deconstruct cell walls using mechanisms that differ considerably from those of the larger, multienzyme complexes (cellulosomes), and high-resolution measurement of the microfibrillar architecture of cell walls suggests that digestion is primarily facilitated by enabling enzyme access to the hydrophobic cellulose face.
Cellobiohydrolase Hydrolyzes Crystalline Cellulose on Hydrophobic Faces*
TLDR
Atomic force microscopy is used to image in real-time the structural changes in Valonia cellulose crystals acted upon by the exocellulase cellobiohydrolase I (CBH I) from Trichoderma reesei, and the observed changes brought about by CBH I action may constitute the first direct visualization supporting the idea that the exoskeleton selectively hydrolyzes the hydrophobic faces of cellulose.
Single-molecule FRET studies of important intermediates in the nucleocapsid-protein-chaperoned minus-strand transfer step in HIV-1 reverse transcription.
TLDR
The single-molecule experiments support the notion that NC-assisted annealing of TAR DNA:TAR RNA may occur through multiple pathways.
Size, shape, and arrangement of native cellulose fibrils in maize cell walls
Higher plant cell walls are the major source of the cellulose used in a variety of industries. Cellulose in plant forms nanoscale fibrils that are embedded in non-cellulosic matrix polymers in the
A biophysical perspective on the cellulosome: new opportunities for biomass conversion.
TLDR
This review focuses on strategies likely to permit improved understanding of the bacterial cellulosome using biophysical approaches, with emphasis on advanced imaging and computational techniques.
Insights on the role of nucleic acid/protein interactions in chaperoned nucleic acid rearrangements of HIV-1 reverse transcription
TLDR
The flow chamber approach involving rapid NC/nucleic acid mixing is used to substantially control aggregation for the NC chaperoned irreversible annealing kinetics of a model TAR DNA hairpin sequence to the complementary TAR RNA hairpin, i.e., to form an extended duplex.
Chemical, ultrastructural and supramolecular analysis of tension wood in Populus tremula x alba as a model substrate for reduced recalcitrance
Biomass is one of the most abundant potential sustainable sources for fuel and material production, however to fully realize this potential an improved understanding of lignocellulosic recalcitrance
Secondary structure and secondary structure dynamics of DNA hairpins complexed with HIV-1 NC protein.
TLDR
Single-molecule fluorescence resonance energy transfer measurements on single immobilized TAR DNA hairpins and hairpin mutants complexed with NC demonstrate that NC shifts the equilibrium secondary structure of TARDNA hairpins from a fully closed conformation to essentially one specific "partially open" conformation.
Label-free, real-time monitoring of biomass processing with stimulated Raman scattering microscopy.
Research into alternative energy has experienced dramatic growth in recent years, which was motivated by both the environmental impact of current fossil fuels and the unstable and uncertain sources
In situ imaging of single carbohydrate-binding modules on cellulose microfibrils.
TLDR
Systematic orientations were observed that are consistent with the CBMs binding to the two opposite hydrophobic faces of the cellulose microfibril, with a well-defined orientation relative to the fiber axis, thus supporting a binding mechanism driven by chemical and structural recognition ofThe cellulose surface.
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