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Identification and characterization of novel esterases from a deep-sea sediment metagenome
A deep-sea sediment metagenomic library was constructed and screened for lipolytic enzymes by activity-based approach. Nine novel lipolytic enzymes were identified, and the amino acid sequencesExpand
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Cloning, expression and characterization of a halotolerant esterase from a marine bacterium Pelagibacterium halotolerans B2T
An esterase PE10 (279 aa) from Pelagibacterium halotolerans B2T was cloned and overexpressed in Escherichia coli Rosetta in a soluble form. The deduced protein was 29.91 kDa and the phylogeneticExpand
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Structure-guided protein engineering increases enzymatic activities of the SGNH family esterases
Background Esterases and lipases hydrolyze short-chain esters and long-chain triglycerides, respectively, and therefore play essential roles in the synthesis and decomposition of ester bonds in theExpand