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BACKGROUND Low cellular uptake and endosomal escape are regarded as major limitations for nonviral gene delivery. In the present study, we use cationic liposomes incorporating fusogenic peptides from glycoprotein H of herpes simplex virus to improve cellular internalization and endosomal release of cationic liposome-DNA complexes. METHODS A synthetic(More)
The development of an automated, high-throughput fractionation procedure to prepare and analyze natural product libraries for drug discovery screening is described. Natural products obtained from plant materials worldwide were extracted and first prefractionated on polyamide solid-phase extraction cartridges to remove polyphenols, followed by(More)
  • Ying Tu, Lin Zhu
  • Journal of controlled release : official journal…
  • 2015
Undesirable physicochemical properties, low tumor targeting, insufficient cell internalization, acquired drug resistance, and severe side effects significantly limit the applications of anticancer drugs. In this study, to improve the tumor targeting and drug efficacy of the poorly water-soluble drug, doxorubicin (DOX), a novel drug delivery platform(More)
Changing polystyrene nanoparticles from three-dimensional spherical shape to two-dimensional disk shape promotes their cell surface binding with significant reduction of cell uptake. As a result of lower cell uptake, nanodisks show very little perturbations on cell functions such as cellular ROS generation, apoptosis and cell cycle progression compared to(More)
The generation of natural product libraries containing column fractions, each with only a few small molecules, using a high-throughput, automated fractionation system, has made it possible to implement an improved dereplication strategy for selection and prioritization of leads in a natural product discovery program. Analysis of databased UPLC-MS-ELSD-PDA(More)
An automated high-throughput method applied to the production and analysis of libraries of natural -products for high-throughput biological screening is described. The production of the library includes solid-phase extraction of crude extracts to remove polyphenols, followed by automated preparative high-performance liquid chromatography (HPLC)(More)
Gene delivery that provides targeted delivery of therapeutic genes to the cells of a lesion enhances therapeutic efficacy and reduces toxic side effects. This process is especially important in cancer therapy when it is advantageous to avoid unwanted damage to healthy, normal cells. Incorporating cancer-specific ligands that recognize receptors(More)
Poor water solubility, low tumor specificity, insufficient cell internalization, and drug resistance are typical among chemotherapy drugs. In this study, the multifunctional micellar nanocarriers containing the PEG2k-pp-PE, a matrix metalloproteinase 2 (MMP2)-labile self-assembling block copolymer, and the TAT-PEG1k-PE, a cell penetrating moiety, were(More)
AIM To increase the solubility and bioavailability of all-trans retinoic acid (ATRA). METHODS Using the principle of lecthin/bile salt mixed micelle to prepared ATRA injection. The best formulation was obtained by the turbidity and three-phase figure. RESULTS ATRA mixed micelles injection is stable. The average size of the mixed micelle is 17.8 nm,(More)
Lipids, one of the major natural products, usually have high biocompatibility and low toxicity. Due to their proper physicochemical properties, they are the most commonly used materials for building modern drug delivery systems, especially nanocarriers. However, to impart new functions or to satisfy special requirements, the lipids can be modified or(More)