Ying Shang

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In this study, a novel universal primer-multiplex-PCR (UP-M-PCR) method adding a universal primer (UP) in the multiplex PCR reaction system was described. A universal adapter was designed in the 5'-end of each specific primer pairs which matched with the specific DNA sequences for each template and also used as the universal primer (UP). PCR products were(More)
— The max-plus linear systems have been studied for almost three decades, however, a well-established system theory on such specific systems is still an ongoing research. The geometric control theory in particular was proposed as the future direction for max-plus linear systems by Cohen et al. This paper reports upon recent investigations on the disturbance(More)
— This paper presents an integrated control strategy solving disturbance decoupling of max-plus linear systems. The classical disturbance decoupling problem (DDP) in geometric control theory means that the controlled outputs will not be changed by any disturbances. The DDP problem has no practical meaning in the context of Max-plus system, since the(More)
— This paper presents a sufficient condition to solve the observation problem in tropical linear event-invariant dynamical systems, where a linear functional of the states can be observed in a finite number of steps using only the information from inputs and outputs. Using the residuation theory, this solvability condition can be easily implemented in(More)
Many genome walking methods based on polymerase chain reaction (PCR) are available, including those with and without restriction enzyme modification. Nevertheless, these methods suffer from low reproducibility, inefficiency, and non-specificity. Here, we present a traceable and efficient PCR strategy: randomly broken fragment PCR with 5' end-directed(More)