Ying Shang

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In this study, a novel universal primer-multiplex-PCR (UP-M-PCR) method adding a universal primer (UP) in the multiplex PCR reaction system was described. A universal adapter was designed in the 5'-end of each specific primer pairs which matched with the specific DNA sequences for each template and also used as the universal primer (UP). PCR products were(More)
— This paper presents a sufficient condition to solve the observation problem in tropical linear event-invariant dynamical systems, where a linear functional of the states can be observed in a finite number of steps using only the information from inputs and outputs. Using the residuation theory, this solvability condition can be easily implemented in(More)
BACKGROUND It is important to achieve the definitive pathogen identification in hospital-acquired pneumonia (HAP), but the traditional culture results always delay the target antibiotic therapy. We assessed the method called quantitative loop-mediated isothermal amplification (qLAMP) as a new implement for steering of the antibiotic decision-making in HAP.(More)
Rannasangpei (RSNP) is used as a therapeutic agent in the treatment of cardiovascular diseases, neurological disorders, and neurodegeneration in China; however, its potential use in the treatment of vascular dementia (VD) was unclear. In this study, our aim was to examine the neuroprotective effect of RSNP in a VD rat model, which was induced by permanent(More)
Many genome walking methods based on polymerase chain reaction (PCR) are available, including those with and without restriction enzyme modification. Nevertheless, these methods suffer from low reproducibility, inefficiency, and non-specificity. Here, we present a traceable and efficient PCR strategy: randomly broken fragment PCR with 5' end-directed(More)
— The max-plus linear systems have been studied for almost three decades, however, a well-established system theory on such specific systems is still an ongoing research. The geometric control theory in particular was proposed as the future direction for max-plus linear systems by Cohen et al. This paper reports upon recent investigations on the disturbance(More)