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Biosynthesis of anthraquinones in cell cultures of the Rubiaceae
TLDR
Findings demonstrate that the 2-C-methyl-D-erythritol 4-phosphate pathway, not the mevalonic acid pathway, is involved in the formation of isopentenyl diphosphate, which constitutes ring C of anthraquinones in the Rubiaceae. Expand
Salicylic Acid Enhances Jaceosidin and Syringin Production in Cell Cultures of Saussurea medusa
TLDR
The results indicate that salicylic acid enhances the production of jaceosidin and syringin which is accompanied by induction of the related phenylpropanoid biosynthetic enzymes. Expand
Identification of fungal oxaloacetate hydrolyase within the isocitrate lyase/PEP mutase enzyme superfamily using a sequence marker‐based method
TLDR
It is proposed that presence of this serine in family members correlates with presence of OAH activity whereas its absence correlates with absence of OAS, and is tested by carrying out a serine mutagenesis study with the OAH from the fungal oxalic acid producer Botrytis cinerea and the O AH active plant petal death protein as test systems. Expand
Cloning of a cDNA encoding 1-deoxy-d-xylulose 5-phosphate synthase from Morinda citrifolia and analysis of its expression in relation to anthraquinone accumulation☆
TLDR
It was shown that the isoprenoid moiety, constituting ring C of anthraquinones in the plant family Rubiaceae, is formed from the MEP pathway, and 1-Deoxy- d -xylulose 5-phosphate synthase (DXS) is suggested to be a regulatory enzyme. Expand
Oxaloacetate Hydrolase, the C–C Bond Lyase of Oxalate Secreting Fungi*
TLDR
It is proposed that cellular uptake of this inhibitor could reduce oxalate production because of the mechanism-based, tight binding OAH inhibitor 3,3-difluorooxaloacetate (Ki = 68 nm). Expand
Structure and function of PA4872 from Pseudomonas aeruginosa, a novel class of oxaloacetate decarboxylase from the PEP mutase/isocitrate lyase superfamily.
TLDR
Genome context of the fourteen sequence family members indicates that the enzyme is used by select group of Gram-negative bacteria to maintain cellular concentrations of bicarbonate and pyruvate; however the decarboxylation activity cannot be attributed to a pathway common to the various bacterial species. Expand
Biosynthesis of anthraquinones in cell cultures of Cinchona 'Robusta' proceeds via the methylerythritol 4-phosphate pathway.
TLDR
To determine the origin of IPP, either formed via the mevalonic acid pathway or the 2-C-methyl-D-erythritol 4-phosphate pathway, the incorporation of [1-13C]glucose into robustaquinone B was studied. Expand
Structure and kinetics of phosphonopyruvate hydrolase from Variovorax sp. Pal2: new insight into the divergence of catalysis within the PEP mutase/isocitrate lyase superfamily.
TLDR
The proposed PPH catalytic mechanism is analogous to that of PEPM but includes activation of a water nucleophile with the loop Thr118 residue and may have favored the open conformation of PPH even when the enzyme was cocrystallized with the oxalate inhibitor. Expand
Diversity of Function in the Isocitrate Lyase Enzyme Superfamily: The Dianthus caryophyllus Petal Death Protein Cleaves α-Keto and α-Hydroxycarboxylic Acids†
TLDR
The work described in this paper was carried out to define the chemical function a new member of the isocitrate lyase enzyme family derived from the flowering plant Dianthus caryophyllus, named the “petal death protein” or “PDP”. Expand
Effects of elicitation on jaceosidin and hispidulin production in cell suspension cultures of Saussurea medusa
TLDR
Silver nitrate and glutathione elicitors increased the production of jaceosidin and hispidulin at optimum dosage and addition time and enhanced phenylalanine ammonialyase (PAL) activity while silver nitrate did not. Expand
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