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Microbial rhodopsins, a diverse group of photoactive proteins found in Archaea, Bacteria, and Eukarya, function in photosensing and photoenergy harvesting and may have been present in the resource-limited early global environment. Four different physiological functions have been identified and characterized for nearly 5,000 retinal-binding photoreceptors,(More)
The purpose of this study was to compare the differences between head nurses expectations and new graduates perceptions of the entry-level skill competency. Subjects included 147 new graduates and 126 head nurses from four schools of nursing and their affiliated medical centers located in northern, central and southern Taiwan. The results showed that among(More)
We investigated a coherent anti-Stokes Raman scattering microscopy with a single-pass picoseconds supercontinuum-seeded optical parametric amplifier (SCOPA). Our SCOPA system is substantially simpler because the pump and Stokes lasers are automatically overlapped.
We report mass resolved photoionization yield spectra of a molecular-beam cooled sample of HN(3) using photoionization mass spectrometry based on high-resolution monochromatized synchrotron radiation. Spectra are reported at mass-to-charge ratios of 14(N(+)), 15(NH(+)), 29(N(2)H(+)), 42(N(3)(+)), and 43(HN(3)(+)) in the region of each ionization threshold.(More)
Matrix-assisted laser desorption ionization (MALDI) has been widely used in the mass analysis of biomolecules; however, there are a lot of debates about the ionization mechanisms. Previous studies have indicated that S1-S1 annihilation might be a key process in the generation of primary ions. This study investigates S1-S1 annihilation by examining the(More)
We recorded photoionization spectra of sulfur atoms in transitions from state (1)D in the range of 75,800-89,500 cm(-1). Dissociation of CS(2) after photolysis at 193 nm produced these sulfur atoms in a singlet excited state; they were then ionized with synchrotron radiation (NSRRC, beamline U9CGM) at resolution of up to 3 cm(-1) and detected with a(More)
The original ion imaging technique has low velocity resolution, and currently, photodissociation is mostly investigated using velocity map ion imaging. However, separating signals from the background (resulting from undissociated excited parent molecules) is difficult when velocity map ion imaging is used for the photodissociation of large molecules (number(More)
RATIONALE Energy pooling has been suggested as the key process for generating the primary ions during ultraviolet matrix-assisted laser desorption/ionization (UV-MALDI). In previous studies, decreases in fluorescence quantum yields as laser fluence increased for 2-aminobenzoic acid, 2,5-dihydroxybenzoic acid (2,5-DHB), and 3-hydroxypicolinic acid were used(More)
Cultural maladjustment of foreign brides is an important social problem. In order to elucidate effective community nursing intervention, a 27 year-old Indonesian bride who had attempted suicide was adopted as a subject presented. In four months of community nursing care, the client's cooperation was won the client's and family's knowledge of suicide was(More)
The ionization mechanism of ultraviolet matrix-assisted laser desorption/ionization (UV-MALDI) was investigated by measuring the total cation intensity (not including sodiated and potasiated ions) as a function of analyte concentration (arginine, histidine, and glycine) in a matrix of 2,4,6-trihydroxyacetophenone (THAP). The total ion intensity increased up(More)