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Real-time quantitative polymerase chain reaction (RT-qPCR), a reliable technique for quantifying gene expression, requires stable reference genes to normalize its data. Salicornia europaea, a stem succulent halophyte with remarkable salt resistance and high capacity for ion accumulation, has not been investigated with regards to the selection of appropriate(More)
In an attempt to determine the adaptation strategy to phosphorous (Pi) deficiency in oilseed rape, comparative proteome analyses were conducted to investigate the differences of metabolic changes in two oilseed rape genotypes with different tolerance to low phosphorus (LP). Generally in either roots or leaves, there existed few low phosphorus (LP)-induced(More)
A 1-kilobase DNA fragment containing the promoter of the bovine prolactin gene was fused to the chloramphenicol acetyltransferase gene and the activity of the promoter was assayed by transfection of the fusion gene into COS-1, HeLa, and GH3 cells. Transcription of the chloramphenicol acetyltransferase gene driven by the prolactin promoter was detected only(More)
BACKGROUND Soil salinity is a major abiotic stress that limits agriculture productivity worldwide. Salicornia europaea is well adapted to extreme saline environments with more than 1,000 mM NaCl in the soil, so it could serve as an important model species for studying halophilic mechanisms in euhalophytes. To obtain insights into the molecular basis of salt(More)
The cuttings of grape (Vitis vinifera Linn.) were exposed to Hoagland's solution containing five different manganese (Mn) concentrations to investigate Mn toxicity and the possible detoxifying responses. Three genotypes (i.e. cultivars Combiner, Jingshou and Shuijing) were used in present study. The results showed that grape species is highly tolerant to(More)
To explore the underlying mechanism for the high tolerance to excess manganese stress in the grape species (Vitis vinifera Linn), we observed the subcellular compartment of Mn element, anatomic and biochemical responses of two grape cultivars (Combier and Shuijin) under excess Mn stress in semi-controlled environmental condition. Grape species exhibited(More)
We compared the growth and physiological responses of cuttings of Populus kangdingensis C. Wang et Tung and P. cathayana Rehder originating from altitudes of 3500 m and 1500 m, respectively, when exposed to three ultraviolet-B (UV-B) radiation regimes: zero UV-B, ambient (4.5 kJ m(-2) day(-1)) and twice-ambient (9 kJ m(-2) day(-1)) biologically effective(More)
Previous studies documented that metal hyperaccumulation armours plants with direct defences against pathogens. In the present study, it was found that high leaf Mn concentrations (<2500 µg g(-1)) induced grapevine resistance to powdery mildew [Uncinula necator (Schw.) Burr]. Manganese delayed pathogen spreading after powdery mildew (PM) inoculation, but(More)
The root-to-shoot transport of manganese (Mn) exhibited intra-specific characters in different grape genotypes. The majority of Mn was stored in the roots of the grape cultivar Jinshou, while it was mainly transferred to the shoots in the cultivar Combier. The aims of the present study was to reveal the complex interplay of gene expression endowing grape a(More)
Grape (cultivar Jinshou) and chestnut rose (cultivar Gui 4) were exposed to excess manganese (Mn) treatments to characterize the physiological basis for Mn tolerance in woody plants. Chestnut rose exhibited a high sensitivity to this environmental constraint whereas grape appeared rather tolerant to Mn excess. Stomatal density and closure rate were affected(More)