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Using voltage clamp and perfusion technique, the types and characteristics of amino acid receptors of carp (Carassius carassius) retinas were studied by expressed response of amphibian oocytes previous injected with the retinal mRNA. The results were as follows: (1) Glu receptor: Diazoxide could potentiated KA induced depolarization, suggesting that some of(More)
The effects of benzyltetrahydropalmatine (BTHP) on delayed rectified K+ currents (Ik) expressed in Xenopus oocytes and Ik of toad (Bufo bufo gargarizans) oocytes were studied. The Ik expressed in Xenopus oocytes was measured after microinjection of mRNA isolated from carp fish (C anratus L.) brains with double -microelectrode voltage clamp technique. The(More)
Two types of GABA receptors, GABAA receptors (approximately 85%) and GABAC-like receptors (approximately 15%), were expressed in Xenopus oocytes after injection of Poly(A)+ RNA with carp (Carassius carassius) brain. Using voltage-clamp technique and pharmacological methods, the effects of zinc on the two receptors were studied. It was found that zinc(More)
Mycobacterium tuberculosis evades innate host immune responses by parasitizing macrophages and causes significant morbidity and mortality around the world. A mycobacterial antigen that can activate dendritic cells (DCs) and elicit effective host innate immune responses will be vital to the development of an effective TB vaccine. The M. tuberculosis genes(More)
Localization of zinc in the carp retina in both light and dark adaptation was investigated using neo-Timm sulfate silver method. The results showed that in light adaptation, zinc was preponderantly present in the outer plexiform layer (OPL). Both terminals of zinc containing photoreceptors and dendrites of bipolar cells usually extend into zinc staining(More)
Oocytes of Bufo bufo gargarizans were used as an expression system for analyzing structure of exogenous membrane protein and their functions. Poly (A)+ mRNA isolated from rat brain was injected into Toad (Bufo bufo gargarizans) oocytes (50 ng/oocyte). Rat brain kainic acid and gamma-aminobutyric acid(GABA) receptors expressed by the injected mRNA were(More)
Injection of total RNA from carp retina induced the formation of a membrane K+ channel in Xenopus oocytes. We found an oligonucleotide complementary to the mRNA coding for a peptide fragment in the K+ channel of drosophila and mice could suppress the K+ channel current in a very specific manner. This provides important information for the cloning of this(More)
Voltage-gated calcium channels, expressed in Xenopus oocytes after injection of rat brain mRNA, were studied by using voltage-clamp technique. The properties of the calcium channels were characterized by barium current (IBa) passed through the channels. All oocytes used in this study were taken from five identified donors. Endogenous voltage--activated(More)
The glutamate receptor and GABA receptor of chicken retina were studied with the expression system of amphibian oocyte. Some important results were obtained: L-glutamate, kainate, AMPA and quisqualate could all induce rapid and smooth depolarizing currents in oocytes. NMDA, L-AP4, trans-ACPD and L-aspartate had no effects. The antagonism of kainate response(More)
Membrane properties of the fully-grown oocytes from toad, Bufo bufo gargarizans, were studied by using voltage-clamp technique. It was found that a sustained outward current was elicited by membrane depolarization to -30 mV or more positive value. The increase of the current was nearly proportional to the degree of depolarization. The peak value of the(More)