Yeong Sheng Lee

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  • Y S Lee
  • Journal of electron microscopy
  • 1993
High resolution electron microscopy (HREM) coupled with energy dispersive X-ray microanalysis were used to investigate the morphogenesis of calcification in 28 explanted porcine bioprosthetic heart valves. Different morphology and compositions of calcific deposits were consistently observed in various valvular structures of all explanted porcine(More)
The gene (lipA) encoding the extracellular lipase and its downstream gene (lipB) from Vibrio vulnificus CKM-1 were cloned and sequenced. Nucleotide sequence analysis and alignments of amino acid sequences suggest that Lip Ais a member of bacterial lipase family I.1 and that LipB is a lipase activator of LipA. The active LipA was produced in recombinant(More)
  • Y S Lee
  • Journal of electron microscopy
  • 1994
High-resolution scanning electron microscopy (HR-SEM) combined with scanning tunneling microscopy (STM) was used to investigate the calcification mechanism occurring in the porcine bioprostheses implanted into the human heart. Eleven explanted porcine heart valves obtained during open heat surgery were prepared for HR-SEM and STM observations. HR-SEM(More)
Two methods have been developed for the detection of DNA immobilized on the surface of microtiter wells. An intercalating dye, 3,6-diaminoacridine, is used in stain and rinse solutions, so that measured absorbance values (450 nm) reflect the sum of DNA-bound and free dye. With diaminoacridine, signal increases of 0.056 +/- 0.010 were achieved on(More)
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