Yeong Cheng Liou

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Insect antifreeze proteins (AFP) are much more effective than fish AFPs at depressing solution freezing points by ice-growth inhibition. AFP from the beetle Tenebrio molitor is a small protein (8.4 kDa) composed of tandem 12-residue repeats (TCTxSxxCxxAx). Here we report its 1.4-A resolution crystal structure, showing that this repetitive sequence(More)
We have previously identified a Thr- and Cys-rich thermal hysteresis (antifreeze) protein (THP) in the beetle Tenebrio molitor that has 10-100 times the freezing point depression activity of fish antifreeze proteins. Because this 8.4 kDa protein is significantly different in its properties from THP preparations previously reported from this insect, a(More)
The yellow mealworm beetle, Tenebrio molitor, produces a number of moderately abundant low molecular weight hemolymph proteins ( approximately 12 kDa) which behave in a similar manner during purification and share antigenic epitopes. The cDNA sequence of the major component (THP12) was determined and the deduced protein sequence was found to be similar to(More)
BACKGROUND THP12 is an abundant and extraordinarily hydrophilic hemolymph protein from the mealworm Tenebrio molitor and belongs to a group of small insect proteins with four highly conserved cysteine residues. Despite their sequence homology to odorant-binding proteins and pheromone-binding proteins, the function of these proteins is unclear. RESULTS The(More)
Pheromones play a vital role in the survival of insects and are used for chemical communication between members of the same species by their olfactory system. The selection and transportation of these lipophilic messengers by carrier proteins through the hydrophilic sensillum lymph in the antennae toward their membrane receptors remains the initial step for(More)
The hyperactive antifreeze protein from the beetle, Tenebrio molitor, is an 8.5-kDa, threonine-rich protein containing 16 Cys residues, all of which are involved in disulfide bonds. When produced by Escherichia coli, the protein accumulated in the supernatant in an inactive, unfolded state. Its correct folding required days or weeks of oxidation at 22 or 4(More)
Hyperactive antifreeze protein from the beetle Tenebrio molitor (TmAFP) was produced in Escherichia coli and purified by gel-permeation chromatography and HPLC. An iodinated derivative was prepared by incubating the 8.5 kDa TmAFP with N-iodosuccinimide. Native and iodinated TmAFP produced two different crystal forms when crystallized using the hanging-drop(More)
As a well-known numerical method, the extragradient method solves numerically the variational inequality VI C,A of finding u ∈ C such that 〈Au, v−u〉 ≥ 0, for all v ∈ C. In this paper,we devote to solve the following hierarchical variational inequality HVI C,A, f Find x̃ ∈ VI C,A such that 〈 I − f x̃, x − x̃〉 ≥ 0, for all x ∈ VI C,A . We first suggest and(More)