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Strain DCY84T, a Gram-stain positive, rod-shaped, aerobic, spore-forming bacterium, motile by means of peritrichous flagella, was isolated from humus soil from Yongin forest in Gyeonggi province, South Korea. Strain DCY84T shared the highest sequence similarity with Paenibacillus barengoltzii KACC 15270T (96.86 %), followed by Paenibacillus timonensis KACC(More)
Microbacterium esteraromaticum was isolated from ginseng field. The β-glucosidase gene (bgp1) from M. esteraromaticum was cloned and expressed in Escherichia coli BL21 (DE3). The bgp1 gene consists of 2,496 bp encoding 831 amino acids which have homology to the glycosyl hydrolase family 3 protein domain. The recombinant β-glucosidase enzyme (Bgp1) was(More)
The ginseng plant (Panax ginseng Meyer) has a large number of active ingredients including steroidal saponins with a dammarane skeleton as well as protopanaxadiol and protopanaxatriol, commonly known as ginsenosides, which have antioxidant, anticancer, antidiabetic, anti-adipocyte, and sexual enhancing effects. Though several discoveries have demonstrated(More)
BACE1, a β secretase candidate enzyme, initiates the Alzheimer's disease (AD) pathogenesis via amyloid β (Aβ) peptide production serving as a potential therapeutic target. Previous experimental evidence suggested that ginsenosides, a key component of Panax ginseng, are effective against AD. In this study, we implemented a molecular modeling method to reveal(More)
Ginsenosides Re and Rg1 were transformed by recombinant β-glucosidase (Bgp1) to ginsenosides Rg2 and Rh1, respectively. The bgp1 gene consists of 2,496 bp encoding 831 amino acids which have homology to the glycosyl hydrolase families 3 protein domain. Using 0.1 mg enzyme ml−1 in 20 mM sodium phosphate buffer at 37°C and pH 7.0, the glucose moiety attached(More)
The ginsenoside-hydrolyzing β-glycosidase (Bgp3) derived from Microbacterium esteraromaticum transformed the major ginsenoside Rb2 to more pharmacologically active minor ginsenosides including compounds Y and K. The bgp3 gene consists of 2,271 bp encoding 756 amino acids which have homology to the glycosyl hydrolase family 3 protein domain. Bgp3 is capable(More)
Strain DCY85T and DCY85-1T, isolated from rhizosphere of ginseng, were rod-shaped, Gram-reaction-negative, strictly aerobic, catalase positive and oxidase negative. 16S rRNA gene sequence analysis revealed that strain DCY85T as well as DCY85-1T belonged to the genus Burkholderia and were closely related to Burkholderia fungorum KACC 12023T (98.1 and 98.0 %(More)
The type strain DCY99T was isolated from soil collected from a ginseng field in Hwacheon, Republic of Korea. Strain DCY99T is Gram-negative, non-spore forming, motile, rod-shaped, and strictly aerobic. The bacteria grow optimally at 25–30 °C and pH 6.0–6.5. Phylogenetically, strain DCY99T is most closely related to Sphingomonas oligophenolica JCM 12082T,(More)
A novel bacterial strain, DCY86T (=KCTC 42053T = JCM 19890T) was isolated from soil of a ginseng field in Yeoncheon province (38°04′00″N 126°57′00″E), Republic of Korea using a serial dilution method. Strain DCY86T was observed to be Gram-stain negative, strictly aerobic, to grow optimally at 25–30 °C, at pH 7–7.5 and on tryptic soya agar medium. The cells(More)
A Gram-staining negative, aerobic, non-motile, non-flagellate, yellow-pigmented, rod-shaped bacterial strain, designated strain DCY67T, was isolated from ginseng field in Republic of Korea. Strain DCY67T contained β-glucosidase activity which converts ginsenoside Rb1 to compound K. Optimum growth of DCY67T occurred at 30 °C and pH 6.0–6.5. Analysis of the(More)