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Development of a multiplex polymerase chain reaction for simultaneous detection of wheat viruses and a phytoplasma in China
For the simultaneous detection and discrimination of these seven pathogens in wheat, a multiplex polymerase chain reaction (M-PCR) method was developed in this study through a series of parameter optimizations and indicates that the method can rapidly, simultaneously and relatively effectively detect BYDV-GAV,-GPV, -PAV, BSMV, WYMV, WDV and WBD. Expand
Detection and Identification of Aster Yellows Group Phytoplasma (16SrI‐C) Associated with Peach Red Leaf Disease
This is the first record of 16SrI-C subgroup phytoplasma occurred in peach tree in China, and phylogenetic analysis and restriction fragment length polymorphism suggested that the phy toplasma associated with such peach red leaf disease was a member of subgroup 16SRI- C. Expand
Brachypodium distachyon is a suitable host plant for study of Barley yellow dwarf virus
The results suggest that B. distachyon is a suitable and promising experimental model plant for the host-BYDV-GAV pathosystem and possibly for other BYDVs. Expand
Psathyrostachys huashanica, a potential resource for resistance to Barley yellow dwarf virus-GAV
It is demonstrated that Psathyrostachys huashanica is highly resistant to BYDV-GAV and possibly has a negative effect on the movement of the viral particles from inoculated leaves to new leaves. Expand
Isolation and characterization of the complete genome of barley yellow dwarf virus-GAV from wheat in Northwestern China
This study provided a detailed description of molecular characterization of BYDV-GAV in Northwestern China based on the complete genome sequences, which increased the understanding of genetic diversity of barley yellow dwarf viruses. Expand
A New Disease of Cherry Plum Tree with Yellow Leaf Symptoms Associated with a Novel Phytoplasma in the Aster Yellows Group
Virtual and actual restriction fragment length polymorphism (RFLP) analysis of the 16S rRNA gene fragment revealed that the phytoplasma was distinguishable from all existing 19 subgroups in the AY group (16SrI) by four restriction sites, Hinf I, Mse I, Sau3A I and Taq I. Expand