Ye G Sukhorukova

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The article presents the information on the modern modifications of Perls' reaction including diaminobenzidine enhancement according to R. Meguro (for various tissues). M.A. Smith (for nervous tissue), S.M. Levine (for detection of iron in oligodendrocytes and myelinated nervous fibers), and our own modification for visualization of the nucleolar iron. The(More)
The distribution of iron ions in the cerebellum of 15 human subjects aged 20-89-years was studied using highly-sensitive variant of Perls' histochemical technique. Increased iron content was found in the white matter and in Purkinje cells. In 10 out of 15 cases examined iron was detected in the nuclei of Purkinje cells, while in some cases iron was found in(More)
This paper describes the study of the neuroepithelial bodies (NEB) in the lungs of adult healthy Wistar rats (n = 12). Using the immunocytochemical reaction demonstrating synaptophysin, NEB and immunopositive nerve terminals approaching them, were visualized. It was found that NEB were the structures constantly presented in the rat lung. In contrast to the(More)
Distribution of iron in the substantia nigra of the human brain (10 men and women aged 27-78 years) was studied using Perls' histochemical method. Iron ions were demonstrated in the nigral neuropil and melanin-containing neurons. For the first time the nuclei of some neurons were found to contain iron accumulations. The intranuclear iron inclusions(More)
In this paper we describe the modern immunohistochemical method detecting the neuronal marker PGP 9.5. The application of this method for the study of the innervation of rat and human heart, the detailed examination of the topography of the cardiac nervous apparatus, as well as their changes in pathological states, is demonstrated. Structural organization(More)
The aim of the study was to investigate the occurrence and intranuclear distribution of Marinesco bodies in substantia nigra neurons of the human brain. Marinesco bodies were identified in substantia nigra sections of 5 men aged 28 to 58 years old using Nissl staining and immunohistochemical detection of ubiquitin--the protein characteristic of this(More)
The aim of the study was to develop the methodologic approaches for the demonstration of the complex synaptic groups (glomeruli) in human cerebellum using synaptophysin immunocytochemistry. The protocols presented in the paper allow to obtain of high qualify prepartaions for conventional light and confocal laser microscopy in which individual glomeruli and(More)
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