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We demonstrate a novel assay for physicochemical extraction and isotachophoresis-based purification of 16S rRNA from whole human blood infected with Pseudomonas putida . This on-chip assay is unique in that the extraction can be automated using isotachophoresis in a simple device with no moving parts, it protects RNA from degradation when isolating from(More)
Capacitive deionization (CDI) is a promising desalination technology, which operates at low pressure, low temperature, requires little infrastructure, and has the potential to consume less energy for brackish water desalination. However, CDI devices consume significantly more energy than the theoretical thermodynamic minimum, and this is at least partly due(More)
This document contains the following supplementary figures and information further describing our bidirectional ITP-based technique for simultaneous purification and fractionation of nucleic acids and proteins from complex biological samples: • Figure S-1: Images of on-chip DNA ITP zones and protein ITP zones • Figure S-2: Experimental setup and procedure •(More)
a b s t r a c t a r t i c l e i n f o Article history: Received 2 June 2016 Received in revised form 9 August 2016 Accepted 15 September 2016 Available online xxxx We report our studies to compare energy consumption of a CDI cell in constant voltage (CV) and constant current (CC) operations, with a focus on understanding the underlying physics of(More)
We report on our efforts to create an on-chip system to simultaneously purify and fractionate nucleic acids and proteins from complex samples using isotachophoresis (ITP). We have developed this technique to simultaneously extract extracellular DNA and proteins from human blood serum samples and deliver these to two separate output reservoirs on a chip. The(More)
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