Yasuo Yoshimi

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A photoresponsive culture surface (PRCS) allowing photocontrol of cell adhesion was prepared with a novel polymer material composed of poly(N-isopropylacrylamide) having spiropyran chromophores as side chains. Cell adhesion of the surface was drastically enhanced by the irradiation with ultraviolet (UV) light (wavelength: 365 nm); after subsequent cooling(More)
The solute diffusive permeability in a thin layer of a molecularly imprinted polymer (MIP) is affected by specific binding of the MIP with a template. This phenomenon, termed the "gate effect," would be widely applicable for the development of novel biomimetic sensors. However, the mechanism underlying the gate effect is not totally understood. We present(More)
The instability of enzymatic glucose sensors has prevented the development of a practical artificial pancreas for diabetic patients. We therefore developed an enzyme-free glucose sensor using the gate effect of a molecularly imprinted polymer (MIP). This sensor has the advantages of improved stability and a simplified manufacturing procedure. An adduct of(More)
Heparin is the most important anticoagulant drug used during surgeries and extracorporeal therapies. Although the blood levels of heparin should be monitored continuously during the procedure to ensure the safety of the patient, there is currently no technique for measuring heparin in real time. This study describes the use of a molecularly imprinted(More)
Based on the enhancement of fluorescein isothiocyanate (FITC) fluorescence caused by binding to proteins, we developed a reagentless immunosensor to directly determine immunoglobulin G (IgG) in human plasma. The fluorescence-enhancement immunoassay (FEI) is used in only limited conditions in spite of its simplicity, because the homogeneous reagent cannot be(More)
In this work, the anodic current at an electrode grafted with a molecularly imprinted polymer (MIP) crosslinked via a combination of hydrophobic ethyleneglycol dimethacrylate (EDMA) and hydrophilic methylene bisacrylamide (MBAA) was found to exhibit enantioselective sensitivity to the phenylalanine template in aqueous solution. An MIP-grafted electrode(More)
Biosensors using enzymes or antibodies are unusable in organic solvents. Therefore, cumbersome and costly chromatographic methods have been mainly used for the analysis in organic solvent. Recently, molecularly imprinted polymers (MIPs) have been expected as the substitutes for the enzymes and the antibodies. MIPs have specific binding sites suitable for(More)
It is known that the diffusive permeability of solutes within a thin layer of molecularly imprinted polymer (MIP) may be affected by specific binding of the MIP with its template molecule. This phenomenon, termed the gate effect, shows promise for the development of novel biomimetic sensors. However, the mechanism underlying this effect is still unclear;(More)
Department of Applied Chemistry, Shibaura Institute of Technology, Tokyo, Japan, Department of Immunology, The Tamil Nadu Dr. MGR Medical University, Chennai, India, Trumed Lifesciences, Chennai, India, The Mary-Yoshio Translational Hexagon (MYTH), Nichi-In Centre for Regenerative Medicine (NCRM), Chennai, India, Loyola-ICAM College of Engineering and(More)