Yasuo Minai

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All CD8+ T cell clones used in this study secreted IL-10, and their proliferation was inhibited by exogenous IL-10. This study addresses the question of whether IL-10 produced by responding T cell clones would inhibit proliferation of the secreting T cells themselves. Anti-IL-10 antibodies enhanced the proliferative response of the CD8+ T cell clones, the(More)
A CD8+ Ts clone 13G2 was established from lymph node cells of bovine alpha s1-casein-primed C57BL/6 mice by in vitro antigenic stimulation followed by maintenance with IL-2-containing medium. The clone suppressed the Ag-induced proliferative responses of CD4+ Th cell clones without detectable cytotoxicity for both APC and responding T cells. The clone was(More)
CD8+ T cell clones produced both interleukin 10 (IL-10) and interferon-gamma (IFN-gamma) when these cells were stimulated with a T-cell-specific mitogen, concanavalin A (Con A). One of these CD8+ T cell clones, 13G2, secreted IFN-gamma at similar levels with calcium ionophore, A23187, as well as by Con A, but IL-10 production by A23187 was less than by Con(More)
CD8+ suppressor T cell (Ts) clone 13G2 produced a soluble suppressive lymphokine, termed the T-cell growth inhibitory factor (TGIF), which suppressed the proliferation of type 1 helper T-cell (Th1) clone 3D20 cells. The specific activity of TGIF was concentrated approximately 66,000-fold (3.7 x 10(6) U/mg of protein) by sequential chromatography from the(More)
In a previous study, we established CD8+ suppressor T cell (Ts) clone 13G2 which produced the suppressive lymphokine, interleukin-10 (IL-10). In this study, we examined what physiological activator could induce both production of IL-10 from 13G2 and the proliferation of 13G2. Both the antigenic stimulation mimicked by the anti-CD3 antibody and the T cell(More)
Highly purified CD8+ T cells were stimulated repeatedly by syngeneic irradiated spleen cells. From separate cloning experiments, we succeeded in isolating two CD8+ clones, 4B4 and D2, which proliferated in response to autologous antigen-presenting cells (APC) completely in the absence of fetal calf serum. A T cell proliferation assay, using congenic strain(More)
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