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Activation of Ubiquitin-Dependent DNA Damage Bypass Is Mediated by Replication Protein A
Summary Replicative DNA damage bypass, mediated by the ubiquitylation of the sliding clamp protein PCNA, facilitates the survival of a cell in the presence of genotoxic agents, but it can alsoExpand
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Ubiquitin-dependent DNA damage bypass is separable from genome replication
Post-replication repair (PRR) is a pathway that allows cells to bypass or overcome lesions during DNA replication. In eukaryotes, damage bypass is activated by ubiquitylation of the replication clampExpand
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A global profile of replicative polymerase usage
Three eukaryotic DNA polymerases are essential for genome replication. Polymerase (Pol) α–primase initiates each synthesis event and is rapidly replaced by processive DNA polymerases: Polɛ replicatesExpand
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Spontaneous mutagenesis in haploid and diploid Saccharomyces cerevisiae.
To obtain insights into the mechanisms of spontaneous mutations in Saccharomyces cerevisiae, we have characterized the genetic alterations that inactivate either the CAN1 gene in haploid cells orExpand
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Polymerase δ replicates both strands after homologous recombination-dependent fork restart
To maintain genetic stability, DNA must be replicated only once per cell cycle, and replication must be completed even when individual replication forks are inactivated. Because fork inactivation isExpand
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Optimisation of the Schizosaccharomyces pombe urg1 Expression System
The ability to study protein function in vivo often relies on systems that regulate the presence and absence of the protein of interest. Two limitations for previously described transcriptionalExpand
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Quantification of DNA-associated proteins inside eukaryotic cells using single-molecule localization microscopy
Development of single-molecule localization microscopy techniques has allowed nanometre scale localization accuracy inside cells, permitting the resolution of ultra-fine cell structure and theExpand
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Spatial separation between replisome‐ and template‐induced replication stress signaling
Polymerase‐blocking DNA lesions are thought to elicit a checkpoint response via accumulation of single‐stranded DNA at stalled replication forks. However, as an alternative to persistent forkExpand
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Error-free RAD52 pathway and error-prone REV3 pathway determines spontaneous mutagenesis in Saccharomyces cerevisiae.
Using the CAN1 gene in haploid cells or heterozygous diploid cells, we characterized the effects of mutations in the RAD52 and REV3 genes of Saccharomyces cerevisiae in spontaneous mutagenesis. TheExpand
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PCNA ubiquitylation ensures timely completion of unperturbed DNA replication in fission yeast
PCNA ubiquitylation on lysine 164 is required for DNA damage tolerance. In many organisms PCNA is also ubiquitylated in unchallenged S phase but the significance of this has not been established.Expand
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