Yasuhito Fujita

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A total of 161 low-G+C-content gram-positive bacteria isolated from whole-crop paddy rice silage were classified and subjected to phenotypic and genetic analyses. Based on morphological and biochemical characters, these presumptive lactic acid bacterium (LAB) isolates were divided into 10 groups that included members of the genera Enterococcus,(More)
Glutamate decarboxy~ase, which is associated with a glutamate-dependent acid-resistance mechanism, was purified from Lactococcus lactis subsp. lactis by a three-step procedure. The specific activity was increased about 114-fold with a yield of 16%. The N-terminal amino acid sequence of the enzyme was determined. The gene encoding th is enzyme was cloned in(More)
Drugs have been implicated in lung injury as a result of direct pharmacological action, persistence or metabolism in the tissue, or via the production of a reactive metabolite or metabolites. The result of this apparent drug-associated injury ranges from cellular dysfunction through to cell death (apoptosis) and alteration of repair mechanisms that are(More)
Bacillus subtilis α-amylase signal peptide, which consists of 33 amino acids, is functional in Escherichia coli cells.Lysine, glutamic acid, leucine, leucyl-leucine, or leucyl-leucyl-leucine was inserted between positions 28 and 29 of the α-amylase signal peptide using site directed mutagenesis. DNAs encoding the wild-type and modified signal peptides were(More)
Recent findings on the functions of roots from the chemical and biological standpoints have strengthened the need for research on the mechanisms of root growth by physical and mathematical approaches. This study was aimed at developing a root growth model, based on dynamic interactions between the root and its ambient environment. As a tool for modelling,(More)
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