Yasuhiro Fujita

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Shikonin derivatives were produced from cell suspension cultures of Lithospermum erythrorhizon for the first time. The results of studies on various culture media showed that the type of nitrogen source in the medium greatly influenced the production of these derivatives. When nitrate was the nitrogen source, stable production of shikonin derivatives by the(More)
DNA markers that allow for identification of resistance genes in rice germplasm have a great advantage in resistance breeding because they can assess the existence of the genes without laborious inoculation tests. Functional markers (FMs), which are designed from functional polymorphisms within the sequence of genes, are unaffected by nonfunctional allelic(More)
The efficiency of glucosylation of hydroquinone by Datura innoxia cell suspension cultures was investigated. The yield of arbutin was 2.4 g/l medium when 10 mM of hydroquinone was added to a suspension culture that was then incubated for 24 h, but the yield decreased at a higher concentration. This decrease, which could not be overcome by changing the(More)
Clostridium cellulovorans 743B was isolated from a wood chip pile and is an anaerobic and mesophilic spore-forming bacterium. This organism degrades native substrates in soft biomass such as corn fiber and rice straw efficiently by producing an extracellular enzyme complex called the cellulosome. Here we report the genome sequence of C. cellulovorans 743B.
A method for quantitative analysis of shikonin derivatives using high pressure liquid chromatography (HPLC) was established. With this method the composition of shikonin derivatives in cultured cells and roots of Lithospermum erythrorhizon (ko-shikon) was compared. The composition of shikonin derivatives produced by cell suspension cultures was similar to(More)
Raman spectroscopy is commonly used in chemistry to identify molecular structure. This technique is a nondestructive analysis and needs no sample preparation. Recently, Raman spectroscopy has been shown to be effective as a multipurpose analytical method for forensic applications. In the present study, blood identification and discrimination between human(More)
The Belle detector was designed and constructed to carry out quantitative studies of rare B-meson decay modes with very small branching fractions using an asymmetric eþe collider operating at the U(4S) resonance, the KEK-B-factory. Such studies require data samples containing B10 B-meson decays. The Belle detector is configured around a 1:5 T(More)
Protoplasts isolated from cell cultures of Lithospermum erythrorhizon divided repeatedly and formed callus colonies. Factors that affect protoplast division are the use of glucose as osmoticum, a new plating method with twin layers of agar-liquid medium, and the culture of protoplasts under the osmolarity lower than that in the isolation solution. When the(More)
As bacteriophages are dependent on the host for multiplication, their infection cycle is expected to be influenced by the host’s physiological state. To elucidate how and which steps of the bacteriophage infection cycle are influenced by changes in the physiological state of the host, we quantitatively compared the infection cycle of lytic RNA bacteriophage(More)
In a recent article Pillai (1990, Ann. Inst. Statist. Math., 42, 157-161) showed that the distribution 1-E~(-x~), 0 < c~ _< 1; 0 _< x, where E~(x) is the Mittag-Lettter function, is infinitely divisible and geometrically infinitely divisible. He also clarified the relation between this distribution and a stable distribution. In the present paper, we(More)